Venkatakrishnan R, Rourke A W
Lab Invest. 1979 Sep;41(3):198-205.
Cardiac myosin was examined during the four pathologic stages of cardiomyopathy in strain BIO 14.6 of Syrian hamsters. It was determined that the Ca2+- and K+-ethylenediaminetetraacetic acid (EDTA)-activated ATPase activities of ventricular myosin were significantly reduced during the final stage of the inherited disease. One- and 2-dimensional gel electrophoresis of myosin samples at all stages failed to yield any evidence for a change in the subunit structure of myosin based on light chain number, molecular weight, and per cent composition. The final stage of the disease was characterized by altered protein metabolism. The rates of synthesis and degradation were both altered in the diseased tissue, and a net loss of myosin resulting from a substantial increase in the rate of degradation.
在叙利亚仓鼠BIO 14.6品系心肌病的四个病理阶段对心肌肌球蛋白进行了检测。结果表明，在该遗传性疾病的最后阶段，心室肌球蛋白的Ca2+和K+ - 乙二胺四乙酸（EDTA）激活的ATP酶活性显著降低。对各阶段肌球蛋白样品进行的一维和二维凝胶电泳均未发现基于轻链数量、分子量和百分比组成的肌球蛋白亚基结构有任何变化的证据。该疾病的最后阶段表现为蛋白质代谢改变。患病组织中合成和降解速率均发生改变，且由于降解速率大幅增加导致肌球蛋白净损失。
