Claerebout E, Smith W D, Pettit D, Geldhof P, Raes S, Geurden T, Vercruysse J
Laboratory of Parasitology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B9820 Merelbeke, Belgium.
Vet Parasitol. 2005 Mar 31;128(3-4):299-307. doi: 10.1016/j.vetpar.2004.12.003. Epub 2005 Jan 22.
The protective capacity of an adult stage Ostertagia ostertagi globin antigen was tested in four vaccination experiments in cattle. In a preliminary experiment, calves were vaccinated three times intraperitoneally with 250 microg globin in Freund's adjuvant and challenged with a trickled infection of 25,000 infective larvae. In three subsequent field studies, calves were vaccinated twice or three times intramuscularly with 80-100 microg globin in Quil A and challenged with a natural gastrointestinal nematode infection on pasture. Higher globin-specific antibody levels were detected in the vaccinated calves than in the control animals in all vaccine trials. In the preliminary experiment, geometric mean cumulative egg counts in the globin group were reduced by 52% and total worm burdens were reduced by 28%, compared to the controls. In the first field trial cumulative faecal egg counts were reduced by 63% in the vaccinated calves. However, the reduction in faecal egg output in these two experiments was not statistically significant and no reduction in faecal egg counts was observed in the vaccinated animals in the two last field trials. In conclusion, vaccination of calves with O. ostertagi globin resulted in highly variable protection levels after challenge infection.
在四项针对牛的疫苗接种实验中，对成年期奥斯特他线虫球蛋白抗原的保护能力进行了测试。在一项初步实验中，给犊牛腹腔注射三次含250微克球蛋白的弗氏佐剂，并接种25,000条感染性幼虫的滴注感染。在随后的三项田间研究中，给犊牛肌肉注射两次或三次含80 - 100微克球蛋白的Quil A，并在牧场上让其感染自然胃肠道线虫。在所有疫苗试验中，接种疫苗的犊牛体内检测到的球蛋白特异性抗体水平均高于对照动物。在初步实验中，与对照组相比，球蛋白组的几何平均累计虫卵数减少了52%，总虫负荷减少了28%。在第一次田间试验中，接种疫苗的犊牛粪便虫卵累计数减少了63%。然而，这两项实验中粪便虫卵产量的减少在统计学上并不显著，并且在最后两项田间试验中，接种疫苗的动物未观察到粪便虫卵数减少。总之，用奥斯特他线虫球蛋白对犊牛进行疫苗接种后，攻击感染后的保护水平差异很大。
