Ruiz Patrícia M Gonçalves, Passos Lygia M Friche, Ribeiro Múcio F Barbosa
Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais (UFMG), C.P. 567, Belo Horizonte, 30123-970 Minas Gerais, Brazil.
Vet Parasitol. 2005 Mar 31;128(3-4):325-31. doi: 10.1016/j.vetpar.2004.11.017. Epub 2004 Dec 24.
Previous studies have shown that one Brazilian Anaplasma marginale isolate presents an inclusion appendage (tail), while other isolates do not present such inclusion. Studies on tick transmission have been carried out with tailless isolates but little is known about transmission of tailed isolates by Boophilus microplus. Two splenectomized calves were experimentally inoculated with the tailed A. marginale isolate. During ascending rickettsemia, B. microplus larvae, free from hemoparasites, were fed on the calves and the resulting nymphs, adult males and engorged females were examined by optic and electronic microscopy. No A. marginale colonies were observed in the gut cells of engorged females and the larvae originated from them did not transmit A. marginale to susceptible calves. In addition, no colonies of A. marginale were seen in the gut cells or in salivary glands of adult males and nymphs. These results suggest that B. microplus is not the biological vector for this tailed isolate.
先前的研究表明，巴西的一株边缘无形体分离株有包涵体附属物（尾巴），而其他分离株则没有这种包涵体。关于蜱传播的研究是用无尾分离株进行的，但对于微小牛蜱传播有尾分离株的情况知之甚少。给两头脾切除的小牛接种了有尾的边缘无形体分离株。在立克次体血症上升期间，将无血寄生虫的微小牛蜱幼虫喂给小牛，然后通过光学显微镜和电子显微镜检查由此产生的若虫、成年雄性蜱和饱血雌性蜱。在饱血雌性蜱的肠道细胞中未观察到边缘无形体菌落，并且由它们产生的幼虫未将边缘无形体传播给易感小牛。此外，在成年雄性蜱和若虫的肠道细胞或唾液腺中也未发现边缘无形体菌落。这些结果表明，微小牛蜱不是这种有尾分离株的生物传播媒介。
