Smith R D, Levy M G, Kuhlenschmidt M S, Adams J H, Rzechula D L, Hardt T A, Kocan K M
Am J Vet Res. 1986 Jan;47(1):127-9.
The tick-borne transmissibility of 2 isolates of Anaplasma marginale was compared. Dermacentor variabilis were exposed to A marginale as nymphs by feeding on 1 of 4 splenectomized calves during periods of ascending parasitemia (maximum 49% to 81% parasitized erythrocytes) induced by injection of a stabilate. Tick-borne transmission was attempted, using 26 to 224 adult ticks within 30 to 220 days after molting. Adult D variabilis did not transmit an Illinois isolate of A marginale in 7 tick-borne transmission experiments (P = 0.0047), including 2 experiments in which calves were inoculated IV with homogenates of adult ticks. In contrast, a Virginia isolate of A marginale was readily transmitted by the same tick colony. Thus, previously reported morphologic and immunologic differences among A marginale isolates may extend to tick-borne transmissibility. The Virginia and Illinois A marginale isolates had an inclusion appendage that was not a marker for tick transmissibility.
对2株边缘无形体的蜱传播能力进行了比较。通过在注射稳定株诱导的寄生虫血症上升期（寄生虫感染红细胞的比例最高为49%至81%）取食4头脾切除小牛中的1头，使变异革蜱若虫暴露于边缘无形体。在蜕皮后30至220天内，使用26至224只成年蜱尝试进行蜱传播。在7次蜱传播实验中，成年变异革蜱未传播伊利诺伊州的边缘无形体分离株（P = 0.0047），其中包括2次给小牛静脉接种成年蜱匀浆的实验。相比之下，同一蜱群很容易传播弗吉尼亚州的边缘无形体分离株。因此，先前报道的边缘无形体分离株之间的形态学和免疫学差异可能延伸至蜱传播能力。弗吉尼亚州和伊利诺伊州的边缘无形体分离株有一个包涵体附属物，但它不是蜱传播的标志物。
