Kim Chong-Kook, Yeon Kyu Jeong, Ban Eunmi, Hyun Myung-Ja, Kim Jin-Ki, Kim Mee-Kyung, Jin Su-Eon, Park Jeong-Sook
Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, San 56-1, Shillim-dong Kwanak-gu, Seoul 151-742, South Korea.
J Pharm Biomed Anal. 2005 Mar 9;37(3):603-9. doi: 10.1016/j.jpba.2004.11.022. Epub 2004 Dec 19.
An improved column switching high performance liquid chromatographic (HPLC) method was developed for determination of cetirizine in human plasma. Plasma samples were prepared by liquid-liquid extraction using methylene chloride. The samples extracted were initially injected into a clean-up Capcell Pak MF C8 column and the peaks of cetirizine and internal standard were separated to an analytical C18 micro-column via column switching device. This analysis showed highly sensitive and selective results. Also, it was successfully applied to evaluate the pharmacokinetics of cetirizine in human volunteers after single oral administration.
建立了一种改进的柱切换高效液相色谱（HPLC）法用于测定人血浆中的西替利嗪。血浆样品采用二氯甲烷液-液萃取法制备。将萃取后的样品首先注入净化的Capcell Pak MF C8柱，通过柱切换装置将西替利嗪和内标物的峰分离至分析型C18微柱。该分析显示出高灵敏度和高选择性的结果。此外，该方法还成功应用于评估单次口服给药后人体志愿者中西替利嗪的药代动力学。
