用于荧光法测定血清中γ-谷氨酰转移酶活性的新底物。
New substrate for fluorometric determination of gamma-glutamyltransferase activity in serum.
作者信息
MacQueen J D, Driscoll R C, Gargiulo R J
出版信息
Clin Chem. 1977 May;23(5):879-81.
PMID:15741
Abstract
We describe the preparation of a new substrate, N-gamma-L-glutamyl-5-aminoisophthalic acid dimethyl ester hydro-chloride, for the fluorometric determination of gamma-glutamyltransferase activity in serum by the "front-surface" technique. Details of the resulting method are provided. The final reaction mixture contains 4 mmol of the substrate per liter of tris(hydroxymethyl)aminomethane (100 mmol/liter) and glycylglycine (75 mmol/liter) at pH 8.2 (25 degrees C).
摘要
我们描述了一种新底物N-γ-L-谷氨酰基-5-氨基间苯二甲酸二甲酯盐酸盐的制备方法，该底物用于通过“前表面”技术荧光测定血清中的γ-谷氨酰转移酶活性。文中给出了所得方法的详细信息。最终反应混合物每升含有4 mmol底物，其中包括三（羟甲基）氨基甲烷（100 mmol/升）和甘氨酰甘氨酸（75 mmol/升），pH值为8.2（25℃）。
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