Hoffmann Nils, Rehm Bernd H A
Institute of Molecular Biosciences, Massey University, Private Bag 11222, Palmerston North, New Zealand.
Biotechnol Lett. 2005 Feb;27(4):279-82. doi: 10.1007/s10529-004-8353-8.
Comparative transcriptional analysis of polyhdroxyalkanoate (PHA) biosynthesis genes with wild type strains and mutants, which lack the intact alternative sigma factor gene rpoN, was performed using semi-quantitative RT-PCR. In Pseudomonas putida and Pseudomonas aeruginosa, phaI and phaF were co-transcribed. PhaF was a negative regulator of transcription of PHA synthase gene phaC1 but did not serve as auto-repressor. However, the alternative sigma factor RpoN is suggested as negative regulator of phaF transcription. In P. putida, phaI-phaF transcription is strongly dependent on nitrogen availability and PHA accumulation, whereas phaF transcription is not. These data suggested a differential regulation of phaF and phaIF. The phaC1 gene transcription occurred almost independently by of RpoN or nitrogen availability in both pseudomonads.
利用半定量逆转录聚合酶链反应（RT-PCR）对聚羟基脂肪酸酯（PHA）生物合成基因与野生型菌株以及缺乏完整替代σ因子基因rpoN的突变体进行了比较转录分析。在恶臭假单胞菌和铜绿假单胞菌中，phaI和phaF是共转录的。PhaF是PHA合酶基因phaC1转录的负调节因子，但不是自抑制因子。然而，替代σ因子RpoN被认为是phaF转录的负调节因子。在恶臭假单胞菌中，phaI-phaF转录强烈依赖于氮的可用性和PHA积累，而phaF转录则不然。这些数据表明phaF和phaIF存在差异调节。在这两种假单胞菌中，phaC1基因转录几乎独立于RpoN或氮的可用性而发生。
