McIntosh G H
Division of Human Nutrition, Commonwealth Scientific and Industrial Research Organization, Adelaide, South Australia.
Nutr Cancer. 1992;17(1):47-55. doi: 10.1080/01635589209514172.
Male Sprague-Dawley rats were fed from weaning low (1-5 ppm) and normal (26-50 ppm) vitamin E diets for 30-34 weeks. Dietary fat was also varied from 5% (Experiment 1) to 20% (Experiments 2 and 3). Intestinal tumors were induced by 1,2-dimethylhydrazine given subcutaneously as 10 weekly doses at 20 mg/kg body wt. Tumor incidence was lower by 30% and burden was 25%-50% lower for low vitamin E rats than for vitamin E-replete rats. This result was independent of the fat content of the diet. In Experiment 3, vitamin E and calcium were assessed for their influence on intestinal tumors at two levels, with dietary vitamin E at 5 and 50 ppm and calcium at 0.2% and 1.0% in a 2 x 2 factorial experiment. The high calcium-low vitamin E diet produced the greatest fall in tumor incidence and burden relative to the other treatments. In this experiment, vitamin E deficiency reduced tumor incidence and calcium supplementation reduced tumor burden, with a significant interaction of the two. However, this group also showed evidence of reduced food intake and kidney change (calcification), which may have confounded the result. This points to a risk associated with this combination of nutrients at these levels in long-term experiments.
将雄性斯普拉格-道利大鼠从断奶开始分别用低剂量（1 - 5 ppm）和正常剂量（26 - 50 ppm）的维生素E饮食喂养30 - 34周。膳食脂肪含量也有所不同，从5%（实验1）到20%（实验2和实验3）。通过皮下注射1,2 - 二甲基肼诱导肠道肿瘤，每周注射10次，剂量为20 mg/kg体重。低维生素E组大鼠的肿瘤发生率比维生素E充足组低30%，肿瘤负荷低25% - 50%。这一结果与饮食中的脂肪含量无关。在实验3中，在一个2×2析因实验中，评估了维生素E和钙在两个水平上对肠道肿瘤的影响，膳食维生素E水平为5 ppm和50 ppm，钙水平为0.2%和1.0%。相对于其他处理，高钙 - 低维生素E饮食导致肿瘤发生率和肿瘤负荷下降幅度最大。在该实验中，维生素E缺乏降低了肿瘤发生率，钙补充降低了肿瘤负荷，二者存在显著交互作用。然而，该组还显示出食物摄入量减少和肾脏变化（钙化）的迹象，这可能混淆了实验结果。这表明在长期实验中，这些营养素水平的这种组合存在风险。
