Zhang Jianling, Han Buxing, Chen Jing, Li Zhonghao, Liu Zhimin, Wu Weize
Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China.
Biotechnol Bioeng. 2005 Feb 5;89(3):274-9. doi: 10.1002/bit.20290.
In this work, a novel route to synthesize biomolecule/metal composite nanospheres is proposed. This method combines the advantages that the silver nanoparticles and bovine serum albumin (BSA) can be precipitated simultaneously from water-in-oil microemulsion by the easy control of CO2 pressure, which was revealed by our high-pressure UV-VIS spectra. The Ag/BSA nanocomposites were successfully prepared using this method. The transmission electronic microscopy (TEM) if the obtained nanocomposites shows that the small-sized Ag nanaoparticles are immobilized by the BSA nanospheres, and the phase structure was characterized by X-ray diffraction (XRD). The Ag/BSA nanocomposites show absorption properties at a wavelength around 435 nm.
在这项工作中，提出了一种合成生物分子/金属复合纳米球的新途径。该方法结合了银纳米颗粒和牛血清白蛋白（BSA）可通过易于控制的二氧化碳压力从油包水微乳液中同时沉淀的优点，这一点已通过我们的高压紫外-可见光谱得到证实。利用该方法成功制备了Ag/BSA纳米复合材料。所获得的纳米复合材料的透射电子显微镜（TEM）显示，小尺寸的Ag纳米颗粒被BSA纳米球固定，并且通过X射线衍射（XRD）对相结构进行了表征。Ag/BSA纳米复合材料在波长约435nm处表现出吸收特性。
