Wang Xiaofeng, Krull Ulrich J
Chemical Sensors Group, Department of Chemical and Physical Sciences, University of Toronto, 3359 Mississauga Road North, Mississauga, ON, Canada L5L 1C6.
Bioorg Med Chem Lett. 2005 Mar 15;15(6):1725-9. doi: 10.1016/j.bmcl.2005.01.030.
Thiazole orange dyes were derivatized with ethylene glycol linkers of various lengths, and were covalently linked to the 5' end of the oligonucleotides after solid-phase synthesis. The labeled oligonucleotides exhibited enhanced fluorescence upon hybridization to complementary DNA sequences at the surfaces of optical fibers, providing for a self-contained labeling strategy. It was determined that the melt temperatures of DNA hybrids using one mixed polypyrimidine base oligonucleotide sequence were dependent on the length of the tethers, and that the melt temperature could be shifted by more than 10 degrees C when tethers were introduced.
噻唑橙染料用不同长度的乙二醇连接基进行衍生化，并在固相合成后与寡核苷酸的5'端共价连接。标记的寡核苷酸在与光纤表面的互补DNA序列杂交时表现出增强的荧光，提供了一种自成体系的标记策略。已确定使用一种混合聚嘧啶碱基寡核苷酸序列的DNA杂交体的解链温度取决于连接链的长度，并且当引入连接链时，解链温度可偏移超过10℃。
