用于基因表达分析的光纤DNA生物传感器微阵列。

A fiber-optic DNA biosensor microarray for the analysis of gene expression.

作者信息

Ferguson J A, Boles T C, Adams C P, Walt D R

机构信息

Max Tishler Laboratory for Organic Chemistry, Department of Chemistry, Tufts University, Medford, MA 02155, USA.

出版信息

Nat Biotechnol. 1996 Dec;14(13):1681-4. doi: 10.1038/nbt1296-1681.

DOI:10.1038/nbt1296-1681

PMID:9634851

Abstract

A fiber-optic biosensor array is described for the simultaneous analysis of multiple DNA sequences. A bundle of optical fibers was assembled with each fiber carrying a different oligonucleotide probe immobilized on its distal end. Hybridization of fluorescently labeled complementary oligonucleotides to the array was monitored by observing the increase in fluorescence that accompanied binding. The approach enables fast (< 10 min) and sensitive (10 nM) detection to multiple DNA sequences simultaneously, with the potential for quantitative hybridization analysis.

摘要

描述了一种用于同时分析多个DNA序列的光纤生物传感器阵列。一束光纤被组装起来，每根光纤在其远端固定有不同的寡核苷酸探针。通过观察结合时伴随的荧光增加来监测荧光标记的互补寡核苷酸与阵列的杂交。该方法能够快速（<10分钟）且灵敏（10 nM）地同时检测多个DNA序列，并具有进行定量杂交分析的潜力。