Palle C, Ottesen B, Fahrenkrug J
Department of Clinical Chemistry, Bispebjerg Hospital, Copenhagen, Denmark.
Regul Pept. 1992 Mar 19;38(2):101-9. doi: 10.1016/0167-0115(92)90048-y.
The occurrence of vasoactive intestinal polypeptide (VIP), peptide histidine methionine (PHM) and peptide histidine valine (PHV) in the human female genital tract was studied by means of radioimmunoassay in combination with gel chromatography. In addition, the effect of PHV on genital smooth muscle activity was investigated in vitro and compared to that of VIP. Immunoreactive VIP, PHM and PHV were present in all regions of the human female genital tract, the highest concentrations being measured in the vagina and the uterine cervix. The peptides displayed similar regional distribution and as expected from the structure of the VIP precursor molecule in which the examined peptides are contained, the molar ratio of VIP to the total PHM/PHV immunoreactivity was close to 1:1. In all regions PHV constituted 50-70% of the total PHM/PHV immunoreactivity indicating that the dibasic conversion site after PHM was uncleaved. VIP and PHV were found to be equipotent relaxants of the smooth muscle from the Fallopian tube and the myometrium. The present study indicates that PHV like PHM and VIP may act as a neurotransmitter in the human female genital tract and thus participate in the local nervous control of the reproductive functions.
采用放射免疫分析法结合凝胶色谱法，研究了血管活性肠肽（VIP）、组氨酸甲硫氨酸肽（PHM）和组氨酸缬氨酸肽（PHV）在女性生殖道中的存在情况。此外，还在体外研究了PHV对生殖器官平滑肌活动的影响，并与VIP进行了比较。免疫反应性VIP、PHM和PHV存在于女性生殖道的所有区域，在阴道和子宫颈中检测到的浓度最高。这些肽表现出相似的区域分布，并且从包含所检测肽的VIP前体分子结构来看，VIP与总PHM/PHV免疫反应性的摩尔比接近1:1。在所有区域，PHV占总PHM/PHV免疫反应性的50 - 70%，表明PHM之后的双碱基转化位点未被切割。发现VIP和PHV对输卵管和子宫肌层的平滑肌具有同等效力的舒张作用。本研究表明，与PHM和VIP一样，PHV可能在女性生殖道中作为神经递质发挥作用，从而参与生殖功能的局部神经控制。
