Ottesen B, Bredkjaer H E, Ekblad E, Krause S, Miskowiak J, Fahrenkrug J
Department of Obstetrics and Gynaecology, Hvidovre Hospital, Copenhagen University, Denmark.
Neuropeptides. 1995 Apr;28(4):227-36. doi: 10.1016/0143-4179(95)90026-8.
Expression of the gene sequence encoding vasoactive intestinal polypeptide (VIP) leads to the synthesis of a 170 amino acid precursor molecule which can be processed to five fragments: preproVIP 22-79, peptide histidine methionine (PHM), or peptide histidine valine (PHV), preproVIP 111-122, VIP and preproVIP 156-170. Using region specific radioimmunoassays and antisera against the functional domains of the VIP precursor in combination with immunocytochemistry and chromatography, the localization, distribution and identity of the preproVIP derived peptides within the human male urogenital tract were investigated. Postmortem as well as fresh tissue specimens were used. All the preproVIP derived peptides were expressed and could be demonstrated in nerve fibres throughout the urogenital tract in close relation to the epithelial lining and in vascular as well as non-vascular smooth muscle. The VIP-related peptide containing fibres were most abundant in the prostate parenchyma and the seminal vesicle. Using double immunostaining, co-localization of the various preproVIP derived peptides could be evidenced. The fact that all preproVIP derived peptides are present in the urogenital tract, should be taken into consideration when the regulatory aspects of neuropeptides in physiological and pathophysiological functions are discussed.
编码血管活性肠肽（VIP）的基因序列的表达会导致一种由170个氨基酸组成的前体分子的合成，该前体分子可被加工成五个片段：前血管活性肠肽22 - 79、肽组氨酸蛋氨酸（PHM）或肽组氨酸缬氨酸（PHV）、前血管活性肠肽111 - 122、血管活性肠肽以及前血管活性肠肽156 - 170。利用针对血管活性肠肽前体功能域的区域特异性放射免疫测定法和抗血清，并结合免疫细胞化学和色谱法，对人类男性泌尿生殖道内源自前血管活性肠肽的肽段的定位、分布和特性进行了研究。使用了尸检组织以及新鲜组织标本。所有源自前血管活性肠肽的肽段均有表达，并且可以在泌尿生殖道的神经纤维中得到证实，这些神经纤维与上皮衬里密切相关，同时也存在于血管和平滑肌以及非血管平滑肌中。含有血管活性肠肽相关肽段的纤维在前列腺实质和精囊中最为丰富。通过双重免疫染色，可以证明各种源自前血管活性肠肽的肽段存在共定位现象。在讨论神经肽在生理和病理生理功能中的调节作用时，应考虑到泌尿生殖道中存在所有源自前血管活性肠肽的肽段这一事实。
