Martínez Ana-Mabel, Williams Trevor, López-Ferber Miguel, Caballero Primitivo
Departamento de Producción Agraria, Universidad Pública de Navarra, Pamplona, Spain.
Appl Environ Microbiol. 2005 Mar;71(3):1668-70. doi: 10.1128/AEM.71.3.1668-1670.2005.
Covert infection with Spodoptera frugiperda multiple nucleopolyhedrovirus, detected by reverse transcription-PCR of virus gene transcripts (ie-0 and polh), was not significantly affected by the presence of an optical brightener (Tinopal UNPA-GX), indicating no change in virus virulence. Detection of the covert infection was dependent on insect life stage and the viral mRNA used for diagnosis.
通过病毒基因转录本(ie-0和多角体蛋白基因)的逆转录聚合酶链反应检测到的草地贪夜蛾多角体病毒隐性感染,不受荧光增白剂(天来宝UNPA-GX)的显著影响,表明病毒毒力没有变化。隐性感染的检测取决于昆虫的生活阶段以及用于诊断的病毒信使核糖核酸。