Wu Guo-Ying, Zhu Ling, Feng Zhen-Qing, Peng Tao, Qi Bing
Department of Oral Medicine, The Stomatology School of Nanjing Medical University. Nanjing 210029,China.
Shanghai Kou Qiang Yi Xue. 2005 Feb;14(1):51-4.
To investigate the roles of hTERT and p53 protein in malignant changes of oral mucosal precancerous lesions and the relations between hTERT and p53.
The expression of hTERTmRNA and p53 were measured using in situ hybridization and immunohistochemical assay in 9 cases of oral mucosal hyperplasia, 11 cases of light dysplasia, 10 cases of medium dysplasia, 9 cases of carcinomas in situ and 11 cases of squamous cell carcinomas. The results were processed by medical photographic system, and analyzed statistically by one-way ANOVA. The relation to hTERT and p53 was analyzed statistically by Pearson correlations.
It was found that from hyperplasia to light dysplasia, medium dysplasia, carcinomas in situ and squamous cell carcinomas, the indexes of positive cell vessels and the value of OD increased, arriving at the apex in squamous cell carcinoma. With phenotypic progression and the degree of dysplasia, hTERT and p53 expression cells extend from basal layer to the keratinous layer. In highly-differentiated squamous cell carcinomas, expression cells were mainly located around the nests. Stained cells dispersed throughout the tumor tissue in low-differentiated squamous cell carcinomas. p53 was undetectable in hyperplasia and light dysplasia, but p53 was observed in 66.67% cases of high dysplasia and 72.72% cases of squamous cell carcinomas. There was not a significant linear correlation between hTERT and p53.
The results suggest that activation of telomerase and p53 play a role in the process of malignant changes of oral mucosal precancerous lesions. Oral squamous cell carcinoma is a disease that has been found in association with many factors.
探讨人端粒酶逆转录酶（hTERT）和p53蛋白在口腔黏膜癌前病变恶变中的作用以及hTERT与p53之间的关系。
采用原位杂交和免疫组化法检测9例口腔黏膜增生、11例轻度不典型增生、10例中度不典型增生、9例原位癌和11例鳞状细胞癌中hTERTmRNA和p53的表达。结果经医学图像系统处理，采用单因素方差分析进行统计学分析。hTERT与p53的关系采用Pearson相关分析进行统计学分析。
发现从增生到轻度不典型增生、中度不典型增生、原位癌和鳞状细胞癌，阳性细胞血管指数和光密度值升高，在鳞状细胞癌中达到峰值。随着表型进展和不典型增生程度，hTERT和p53表达细胞从基底层延伸至角质层。在高分化鳞状细胞癌中，表达细胞主要位于巢周。在低分化鳞状细胞癌中，染色细胞分散于整个肿瘤组织。在增生和轻度不典型增生中未检测到p53，但在66.67%的高度不典型增生病例和72.72%的鳞状细胞癌病例中观察到p53。hTERT与p53之间无显著线性相关性。
结果表明端粒酶激活和p53在口腔黏膜癌前病变恶变过程中起作用。口腔鳞状细胞癌是一种与多种因素相关的疾病。
