Peskan-Berghöfer Tatjana, Neuwirth Jana, Kusnetsov Victor, Oelmüller Ralf
Institute of General Botany and Plant Physiology, Friedrich-Schiller-University Jena, Dornburgerstr. 159, 07743 Jena, Germany.
Planta. 2005 Mar;220(5):737-46. doi: 10.1007/s00425-004-1393-4. Epub 2005 Jan 27.
The role of the heterotrimeric G-protein beta-subunit in plant development was studied in transgenic tobacco (Nicotiana tabacum L.) plants with reduced beta-subunit levels due to the antisense expression of the beta-subunit mRNA. The antisense plants had aberrant anther shape and produced non-germinating pollen. The anthers were sporadically transformed to petals, whereas other floral organs were not affected. The pollen grains were smaller than the wild-type pollen and had abnormal cell walls. The architecture of mature antisense plants was altered. The plants had long branched panicles and short stems. These data suggest that the beta-subunit of the plant heterotrimeric G-proteins is involved in the regulation of the reproductive phase of the tobacco life cycle, particularly in stamen development and pollen maturation.
通过反义表达β亚基mRNA，在β亚基水平降低的转基因烟草（Nicotiana tabacum L.）植株中研究了异源三聚体G蛋白β亚基在植物发育中的作用。反义植株的花药形状异常，产生的花粉不能萌发。花药偶尔会转变为花瓣，而其他花器官未受影响。花粉粒比野生型花粉小，细胞壁异常。成熟反义植株的结构发生了改变。植株具有长的分枝圆锥花序和短茎。这些数据表明，植物异源三聚体G蛋白的β亚基参与烟草生命周期生殖阶段的调控，特别是在雄蕊发育和花粉成熟过程中。
