Jo Y S, Kim D K, Muhammed M
Materials Chemistry Division, Royal Institute of Technology, Stockholm, SE, 100 44, Sweden.
J Mater Sci Mater Med. 2004 Dec;15(12):1291-5. doi: 10.1007/s10856-004-5737-1.
A method to construct synchronous delivery systems via direct self-assembly of Au nanoparticles on the poly[(N-isopropylacrylamide-r-acrylamide)-b-L-lactic acid] (PNAL) nanospheres has been presented in this paper. To achieve amphiphilic diblock terpolymer, hydrophobic poly (L-lactic acid) (PLLA) block was added to poly(N-isopropylacrylamide- r-acrylamide) (PNA) block via Michel-type addition reaction. Lower critical solubility temperature (LCST) was modulated at 35.6 degrees C which is close to the body temperature, but higher than poly(N-isopropylacrylamide) (PNIPAAm) homopolymer by controlling the ratio between isopropylacrylamide (IPAAm) monomers and acrylamide (AAm) monomers. Using this amphiphilic diblock terpolymer, PNAL nanospheres were fabricated by emulsion/evaporation technique followed by direct self-assembly of Au nanoparticles on the PNAL nanospheres due to the high affinity of amino groups donated from PNA block. The 'core' site of Au@PNAL nanospheres can load various lyphophilic drugs. Moreover, Au nanoparticles in the 'shell' domain of PNAL nanospheres give optimal environment to conjugate various biomolecules. Therefore, it is expected that Au@PNAL hybrid nanospheres can be utilized in synchronous delivery of both biomolecules in the 'shell' domain and various therapeutic drugs in the 'core' domain.
本文提出了一种通过金纳米颗粒在聚(N-异丙基丙烯酰胺-r-丙烯酰胺)-b-L-乳酸纳米球上直接自组装构建同步递送系统的方法。为了获得两亲性二嵌段三元共聚物，通过迈克尔型加成反应将疏水性聚(L-乳酸)(PLLA)嵌段添加到聚(N-异丙基丙烯酰胺-r-丙烯酰胺)(PNA)嵌段上。通过控制异丙基丙烯酰胺(IPAAm)单体与丙烯酰胺(AAm)单体之间的比例，将低临界溶解温度(LCST)调节至35.6℃，该温度接近体温，但高于聚(N-异丙基丙烯酰胺)(PNIPAAm)均聚物。使用这种两亲性二嵌段三元共聚物，通过乳液/蒸发技术制备PNAL纳米球，随后由于PNA嵌段提供的氨基具有高亲和力，金纳米颗粒在PNAL纳米球上直接自组装。Au@PNAL纳米球的“核心”部位可以负载各种亲脂性药物。此外，PNAL纳米球“壳”域中的金纳米颗粒为共轭各种生物分子提供了最佳环境。因此，预计Au@PNAL杂化纳米球可用于同步递送“壳”域中的生物分子和“核心”域中的各种治疗药物。
