[用于鉴定A组轮状病毒VP4基因的逆转录-聚合酶链反应优化及其诊断效率评估]
[Optimization of RT-PCR for the identification of VP4 gene of group A rotaviruses and evaluation of its diagnostic efficiency].
作者信息
Fedorova O F, Novikova N A, Epifanova N V, Lukovnikova L B, Kniagina O N, Gracheva E V, Bogacheva E M
出版信息
Vopr Virusol. 2005 Jan-Feb;50(1):39-41.
A method is suggested for the detection of the human rotavirus VP4 gene by RT-PCR. The method is universal for all [P] types of group A rotavirus in clinical samples. As against RNA electrophoresis and ELISA, it provides for a more accurate detection of rotaviruses by 18.8 and 26.5%, respectively. It was established by RT-PCR in February, 2002, in Nizhny Novgorod, that rotaviruses were responsible for 67% of acute gastroenteritis in hospitalized children.
提出了一种通过逆转录聚合酶链反应（RT-PCR）检测人轮状病毒VP4基因的方法。该方法对临床样本中所有A组轮状病毒的[P]型均通用。与RNA电泳和酶联免疫吸附测定（ELISA）相比，它分别能更准确地检测出轮状病毒，准确率提高了18.8%和26.5%。2002年2月在下诺夫哥罗德通过RT-PCR确定，轮状病毒是住院儿童急性肠胃炎的病因，占比67%。
Zotero 插件