• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

在干扰素-β基因敲除小鼠的过敏性气道炎症小鼠模型中使用含CpG基序的局部治疗。

Local therapy with CpG motifs in a murine model of allergic airway inflammation in IFN-beta knock-out mice.

作者信息

Matheu Victor, Treschow Alexandra, Teige Ingrid, Navikas Vaidrius, Issazadeh-Navikas Shohreh

机构信息

Section of Medical Inflammation Research, Department of Cell & Molecular Biology; Lund University; Sweden.

出版信息

Respir Res. 2005 Mar 5;6(1):25. doi: 10.1186/1465-9921-6-25.

DOI:10.1186/1465-9921-6-25
PMID:15748290
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC555575/
Abstract

BACKGROUND

CpG oligodeoxynucleotides (CpG-ODN) are capable of inducing high amounts of type I IFNs with many immunomodulatory properties. Furthermore, type-I IFNs have been proposed to play a key role in mediating effects of CpG-ODN. The precise role of IFN-beta in the immunomodulatory effects of CpG-ODN is not known.

OBJECTIVE

Here, we aimed to elucidate the role of IFN-beta in the anti-allergic effect of CpG motifs.

METHODS

We assessed the immune response in OVA-primed/OVA-challenged IFN-beta knockout (-/-) mice compared to wild type (WT) control, after intranasal and systemic treatment with synthetic CpG motifs.

RESULTS

Vaccination with CpG-ODN reduced the number of cells in airways of OVA-sensitized WT but not IFN-beta-/- mice. Although airway eosinophilia was reduced in both treated groups, they were significantly higher in IFN-beta-/- mice. Other inflammatory cells, such as lymphocytes and macrophages were enhanced in airways by CpG treatment in IFN-beta-/- mice. The ratio of IFN-gamma/IL-4 cytokines in airways was significantly skewed to a Th1 response in WT compared to IFN-beta-/- group. In contrast, IL-4 and IgE were reduced with no differences between groups. Ag-specific T-cell proliferation, Th1-cytokines such as IFN-gamma, IL-2 and also IL-12 were significantly lower in IFN-beta-/- mice. Surprisingly, we discovered that intranasal treatment of mice with CpG-ODN results in mild synovitis particularly in IFN-beta-/- mice.

CONCLUSION

Our results indicate that induction of Th1 response by therapy with CpG-ODN is only slightly and partially dependent on IFN-beta, while IFN-beta is not an absolute requirement for suppression of airway eosinophilia and IgE. Furthermore, our finding of mild synovitis is a warning for possible negative effects of CpG-ODN vaccination.

摘要

背景

CpG寡脱氧核苷酸（CpG-ODN）能够诱导产生大量具有多种免疫调节特性的I型干扰素。此外，I型干扰素被认为在介导CpG-ODN的作用中起关键作用。干扰素-β在CpG-ODN免疫调节作用中的具体作用尚不清楚。

目的

在此，我们旨在阐明干扰素-β在CpG基序抗过敏作用中的作用。

方法

在用合成CpG基序进行鼻内和全身治疗后，我们评估了卵清蛋白致敏的干扰素-β基因敲除（-/-）小鼠与野生型（WT）对照相比的免疫反应。

结果

用CpG-ODN疫苗接种可减少卵清蛋白致敏的WT小鼠气道中的细胞数量，但对干扰素-β-/-小鼠无效。虽然两个治疗组的气道嗜酸性粒细胞增多均有所减少，但在干扰素-β-/-小鼠中显著更高。CpG治疗可使干扰素-β-/-小鼠气道中的其他炎症细胞（如淋巴细胞和巨噬细胞）增多。与干扰素-β-/-组相比，WT小鼠气道中干扰素-γ/白细胞介素-4细胞因子的比例显著偏向于Th1反应。相反，白细胞介素-4和免疫球蛋白E减少，两组之间无差异。在干扰素-β-/-小鼠中，抗原特异性T细胞增殖、Th1细胞因子（如干扰素-γ、白细胞介素-2以及白细胞介素-12）显著降低。令人惊讶的是，我们发现用CpG-ODN对小鼠进行鼻内治疗会导致轻度滑膜炎，尤其是在干扰素-β-/-小鼠中。

结论

我们的结果表明，用CpG-ODN治疗诱导Th1反应仅轻微且部分依赖于干扰素-β，而干扰素-β并非抑制气道嗜酸性粒细胞增多和免疫球蛋白E的绝对必需条件。此外，我们发现的轻度滑膜炎警示了CpG-ODN疫苗接种可能存在的负面影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bde/555575/b75494a5dbb6/1465-9921-6-25-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bde/555575/c472251a8de7/1465-9921-6-25-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bde/555575/1526837f02d0/1465-9921-6-25-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bde/555575/e08d9d9b3e25/1465-9921-6-25-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bde/555575/2a2686760307/1465-9921-6-25-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bde/555575/2c5a2bab0d43/1465-9921-6-25-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bde/555575/b75494a5dbb6/1465-9921-6-25-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bde/555575/c472251a8de7/1465-9921-6-25-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bde/555575/1526837f02d0/1465-9921-6-25-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bde/555575/e08d9d9b3e25/1465-9921-6-25-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bde/555575/2a2686760307/1465-9921-6-25-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bde/555575/2c5a2bab0d43/1465-9921-6-25-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bde/555575/b75494a5dbb6/1465-9921-6-25-6.jpg

相似文献

1
Local therapy with CpG motifs in a murine model of allergic airway inflammation in IFN-beta knock-out mice.在干扰素-β基因敲除小鼠的过敏性气道炎症小鼠模型中使用含CpG基序的局部治疗。
Respir Res. 2005 Mar 5;6(1):25. doi: 10.1186/1465-9921-6-25.
2
Modulation of murine allergic rhinosinusitis by CpG oligodeoxynucleotides.CpG 寡脱氧核苷酸对小鼠变应性鼻鼻窦炎的调节作用
Laryngoscope. 2002 Oct;112(10):1819-26. doi: 10.1097/00005537-200210000-00021.
3
CpG-ODN inhibits airway inflammation at effector phase through down-regulation of antigen-specific Th2-cell migration into lung.CpG寡脱氧核苷酸通过下调抗原特异性Th2细胞向肺内的迁移，在效应阶段抑制气道炎症。
Int Immunol. 2008 Feb;20(2):259-66. doi: 10.1093/intimm/dxm138. Epub 2007 Dec 21.
4
CpG ODN mediated prevention from ovalbumin-induced anaphylaxis in mouse through B cell pathway.CpG寡脱氧核苷酸通过B细胞途径介导预防小鼠卵清蛋白诱导的过敏反应。
Int Immunopharmacol. 2008 Feb;8(2):351-61. doi: 10.1016/j.intimp.2007.10.019. Epub 2007 Nov 20.
5
Vaccinations with T-helper type 1 directing adjuvants have different suppressive effects on the development of allergen-induced T-helper type 2 responses.使用1型辅助性T细胞导向佐剂进行疫苗接种，对变应原诱导的2型辅助性T细胞反应的发展具有不同的抑制作用。
Clin Exp Allergy. 2005 Aug;35(8):1003-13. doi: 10.1111/j.1365-2222.2005.02287.x.
6
Immunotherapy of Chenopodium album induced asthma by intranasal administration of CpG oligodeoxynucleotides in BALB/c mice.通过在BALB/c小鼠中鼻内给予CpG寡脱氧核苷酸对藜诱导的哮喘进行免疫治疗。
Iran J Immunol. 2008 Mar;5(1):57-63.
7
An indispensable role of type-1 IFNs for inducing CTL-mediated complete eradication of established tumor tissue by CpG-liposome co-encapsulated with model tumor antigen.1型干扰素在通过与模型肿瘤抗原共包封的CpG脂质体诱导CTL介导的已建立肿瘤组织完全根除中起不可或缺的作用。
Int Immunol. 2006 Mar;18(3):425-34. doi: 10.1093/intimm/dxh381. Epub 2006 Jan 13.
8
CpG motifs of bacterial DNA exert protective effects in mouse models of IBD by antigen-independent tolerance induction.细菌DNA的CpG基序通过非抗原依赖性耐受诱导在炎症性肠病小鼠模型中发挥保护作用。
Gastroenterology. 2009 Jan;136(1):278-87. doi: 10.1053/j.gastro.2008.09.022. Epub 2008 Sep 25.
9
Cytosine-phosphate-guanine motifs fail to promote T-helper type 1-polarized responses in human neonatal mononuclear cells.胞嘧啶-磷酸-鸟嘌呤基序无法促进人类新生儿单核细胞中1型辅助性T细胞极化反应。
Clin Exp Allergy. 2005 Mar;35(3):358-66. doi: 10.1111/j.1365-2222.2005.02187.x.
10
Long term prevention of allergic lung inflammation in a mouse model of asthma by CpG oligodeoxynucleotides.CpG寡脱氧核苷酸对哮喘小鼠模型过敏性肺部炎症的长期预防作用
J Immunol. 1999 May 15;162(10):6284-93.

引用本文的文献

1
Remission of collagen-induced arthritis through combination therapy of microfracture and transplantation of thermogel-encapsulated bone marrow mesenchymal stem cells.通过微骨折与热凝胶包裹的骨髓间充质干细胞移植联合治疗实现胶原诱导性关节炎的缓解。
PLoS One. 2015 Mar 16;10(3):e0120596. doi: 10.1371/journal.pone.0120596. eCollection 2015.
2
Impact on allergic immune response after treatment with vitamin A.维生素 A 治疗对过敏免疫反应的影响。
Nutr Metab (Lond). 2009 Oct 23;6:44. doi: 10.1186/1743-7075-6-44.
3
Similar response in male and female B10.RIII mice in a murine model of allergic airway inflammation.

本文引用的文献

1
CpG motifs in bacterial DNA delay apoptosis of neutrophil granulocytes.细菌DNA中的CpG基序可延迟中性粒细胞的凋亡。
FASEB J. 2004 Nov;18(14):1776-8. doi: 10.1096/fj.04-2048fje. Epub 2004 Sep 2.
2
Oligodeoxynucleotides containing CpG motifs can induce T cell-dependent arthritis in rats.含有CpG基序的寡脱氧核苷酸可诱导大鼠发生T细胞依赖性关节炎。
Arthritis Rheum. 2004 Jan;50(1):297-304. doi: 10.1002/art.11488.
3
CD1-dependent regulation of chronic central nervous system inflammation in experimental autoimmune encephalomyelitis.
在变应性气道炎症的小鼠模型中，B10.RIII 雌雄小鼠的反应相似。
Inflamm Res. 2010 Apr;59(4):263-9. doi: 10.1007/s00011-009-0094-7. Epub 2009 Sep 25.
4
Immune modulatory oligonucleotides in the prevention and treatment of allergen-induced eustachian tube dysfunction in the animal model.
Curr Allergy Asthma Rep. 2008 Nov;8(6):508-12. doi: 10.1007/s11882-008-0093-8.
实验性自身免疫性脑脊髓炎中CD1依赖性对慢性中枢神经系统炎症的调节
J Immunol. 2004 Jan 1;172(1):186-94. doi: 10.4049/jimmunol.172.1.186.
4
Less sensitivity for late airway inflammation in males than females in BALB/c mice.在BALB/c小鼠中，雄性对晚期气道炎症的敏感性低于雌性。
Scand J Immunol. 2003 Jun;57(6):562-7. doi: 10.1046/j.1365-3083.2003.01269.x.
5
IFN-beta gene deletion leads to augmented and chronic demyelinating experimental autoimmune encephalomyelitis.干扰素-β基因缺失导致实验性自身免疫性脑脊髓炎加重并慢性脱髓鞘。
J Immunol. 2003 May 1;170(9):4776-84. doi: 10.4049/jimmunol.170.9.4776.
6
Intranasal immunotherapy is more effective than intradermal immunotherapy for the induction of airway allergen tolerance in Th2-sensitized mice.对于诱导Th2致敏小鼠的气道变应原耐受性，鼻内免疫疗法比皮内免疫疗法更有效。
J Immunol. 2003 Apr 1;170(7):3898-905. doi: 10.4049/jimmunol.170.7.3898.
7
Upregulation of B7 molecules (CD80 and CD86) and exacerbated eosinophilic pulmonary inflammatory response in mice lacking the IFN-beta gene.在缺乏IFN-β基因的小鼠中，B7分子（CD80和CD86）上调，嗜酸性粒细胞性肺部炎症反应加剧。
J Allergy Clin Immunol. 2003 Mar;111(3):550-7. doi: 10.1067/mai.2003.112.
8
IFN-alpha beta promote priming of antigen-specific CD8+ and CD4+ T lymphocytes by immunostimulatory DNA-based vaccines.干扰素αβ可促进基于免疫刺激DNA的疫苗引发抗原特异性CD8+和CD4+ T淋巴细胞。
J Immunol. 2002 May 15;168(10):4907-13. doi: 10.4049/jimmunol.168.10.4907.
9
The genetic control of sialadenitis versus arthritis in a NOD.QxB10.Q F2 cross.在NOD.QxB10.Q F2杂交中涎腺炎与关节炎的遗传控制
Eur J Immunol. 2002 Jan;32(1):243-50. doi: 10.1002/1521-4141(200201)32:1<243::AID-IMMU243>3.0.CO;2-X.
10
Distinct CpG oligonucleotide sequences activate human gamma delta T cells via interferon-alpha/-beta.不同的CpG寡核苷酸序列通过α/β干扰素激活人γδ T细胞。
Eur J Immunol. 2001 Dec;31(12):3525-34. doi: 10.1002/1521-4141(200112)31:12<3525::aid-immu3525>3.0.co;2-5.