Department of Clinical Sciences-Division IV; Lund University, Lund, Sweden.
Nutr Metab (Lond). 2009 Oct 23;6:44. doi: 10.1186/1743-7075-6-44.
Vitamin A may have some influence on the immune system, but the role in allergy modulation is still unclear.
To clarify whether high levels of retinoic acid (RA) affects allergic response in vivo, we used a murine experimental model of airway allergic disease.
Ovalbumin (OVA)-immunization/OVA-challenge (OVA/OVA) and house dust mite (HDM)-immunization/HDM-challenge (HDM/HDM) experimental murine models of allergic airway disease, using C57Bl.10/Q groups of mice (n = 10) treated subcutaneously with different concentrations of all-trans RA (0, 50, 500 and 2,500 ug) every 2-days were used to assess the allergic immune response.
Levels of total and specific-IgE in sera were increased in all groups of RA treated OVA/OVA and HDM/HDM mice. Percentage and total amount of recruited eosinophil in airways by bronchoalveolar lavage fluid (BALF) were significantly enhanced in groups treated with 50, 500 and 2,500 ug of RA compared to non-treated mice. However, the group of mice treated with 2,500 ug had less eosinophil recruitment than the other two groups (50 and 500 ug). In parallel, levels of IL-5 and total IgE in BALF were also significantly diminished in the group treated with 2,500 ug compared to the other 2 groups (50 and 500 ug). Finally, total lung resistance was decreased in group treated with 2,500 ug compared to non-treated mice.
Our results suggest that retinoic acid directly enhances allergic response in vivo, but in higher doses may produce of immune suppression.
维生素 A 可能对免疫系统有一定影响，但在过敏调节中的作用仍不清楚。
为了阐明高浓度视黄酸 (RA) 是否会影响体内过敏反应，我们使用了一种气道过敏性疾病的小鼠实验模型。
使用卵清蛋白 (OVA) 免疫/OVA 攻毒 (OVA/OVA) 和屋尘螨 (HDM) 免疫/HDM 攻毒 (HDM/HDM) 过敏性气道疾病的实验小鼠模型，用 C57Bl.10/Q 组小鼠 (n = 10) 经皮给予不同浓度的全反式 RA(0、50、500 和 2500μg)，每 2 天一次，评估过敏免疫反应。
RA 处理的 OVA/OVA 和 HDM/HDM 小鼠血清中的总 IgE 和特异性 IgE 水平均升高。经 RA(50、500 和 2500μg)处理的小鼠气道中支气管肺泡灌洗液 (BALF) 中募集的嗜酸性粒细胞的百分比和总量明显高于未处理的小鼠。然而，与其他两组(50 和 500μg)相比，用 2500μg 处理的小鼠组中嗜酸性粒细胞的募集量较少。同时，BALF 中 IL-5 和总 IgE 的水平在 2500μg 处理组也明显低于其他两组(50 和 500μg)。最后，与未处理的小鼠相比，用 2500μg 处理的小鼠的总肺阻力降低。
我们的结果表明，视黄酸直接增强体内过敏反应，但在较高剂量下可能产生免疫抑制。
