Li Shuang, Doss Jason C, Hardee Elizabeth J, Quock Raymond M
Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, Pullman, WA 99164-6534, USA.
Brain Res. 2005 Mar 15;1038(1):113-7. doi: 10.1016/j.brainres.2005.01.015.
The second messenger cyclic GMP (cGMP) plays a role in the anxiolytic-like behavioral response of mice to nitrous oxide (N2O). This study was conducted to determine whether this behavioral effect of N2O is affected by inhibition of cGMP-dependent protein kinase (PKG). N2O-induced behavior in the light/dark exploration test was significantly attenuated by the PKG inhibitors H-8 and Rp-8-pCPT-cGMPS but not Rp-8-pCPT-cAMPS, an inhibitor of cAMP-dependent protein kinase. These findings implicate PKG in the mediation or modulation of the anxiolytic-like behavioral response to N2O.
第二信使环磷酸鸟苷（cGMP）在小鼠对一氧化二氮（N₂O）的抗焦虑样行为反应中发挥作用。本研究旨在确定N₂O的这种行为效应是否受cGMP依赖性蛋白激酶（PKG）抑制的影响。在明暗箱探索试验中，PKG抑制剂H-8和Rp-8-pCPT-cGMPS可显著减弱N₂O诱导的行为，但cAMP依赖性蛋白激酶抑制剂Rp-8-pCPT-cAMPS则无此作用。这些发现表明PKG参与介导或调节对N₂O的抗焦虑样行为反应。
