用鼠伤寒沙门氏菌活疫苗预防绵羊流产沙门氏菌引起的绵羊流产

Prophylaxis of Salmonella abortus ovis-induced abortion of sheep by a Salmonella typhimurium live vaccine.

作者信息

Linde K, Bondarenko V, Sviridenko V

机构信息

Institute of Medical Microbiology and Epidemiology, Leipzig University, Germany.

出版信息

Vaccine. 1992;10(5):337-40. doi: 10.1016/0264-410x(92)90374-s.

DOI:10.1016/0264-410x(92)90374-s

PMID:1574918

Abstract

A Salmonella typhimurium live vaccine with optimal level of attenuation for sheep, constructed by means of 'metabolic drift' mutations, was tested for its efficacy in preventing Salmonella abortus ovis-induced abortions. In two field trials in Kirgiziya, 78,000 to 100,000 first delivery sheep received a fully tolerated single dose of 10(9) c.f.u. live vaccine 2 months before to 4 months after insemination. Alternatively, they were immunized twice with commercial inactivated S. abortus ovis vaccine, or they served as non-immunized controls. The S. abortus ovis-induced abortion frequency in the controls was greater than or equal to 30%, in sheep immunized with inactivated vaccine greater than or equal to 11%. In flocks immunized with live vaccine, the S. abortus ovis-induced abortion frequency did not exceed 0.1%. Thus, use of 'metabolic drift' mutations for construction of stable vaccine strains optimally attenuated for the particular host species proved to be relevant to practice.

摘要

通过“代谢漂移”突变构建的对绵羊具有最佳减毒水平的鼠伤寒沙门氏菌活疫苗，对其预防绵羊流产沙门氏菌引起的流产的效果进行了测试。在吉尔吉斯斯坦的两项田间试验中，78,000至100,000只初产绵羊在授精前2个月至授精后4个月接受了10⁹ c.f.u.的单剂量完全可耐受的活疫苗。或者，它们用市售的灭活绵羊流产沙门氏菌疫苗免疫两次，或者作为未免疫对照。对照中绵羊流产沙门氏菌引起的流产频率大于或等于30%，用灭活疫苗免疫的绵羊中大于或等于11%。在用活疫苗免疫的羊群中，绵羊流产沙门氏菌引起的流产频率不超过0.1%。因此，利用“代谢漂移”突变构建对特定宿主物种具有最佳减毒效果的稳定疫苗株被证明与实际应用相关。