Mukkur T K, Walker K H, Baker P, Jones D
CSIRO Division of Animal Health, McMaster Laboratory, Glebe, N.S.W.
Comp Immunol Microbiol Infect Dis. 1995 Jan;18(1):27-39. doi: 10.1016/0147-9571(93)e0012-5.
Following the development of a suitable formulation capable of inhibiting intestinal proteolytic activity, the total anti-lipopolysaccharide (LPS) and anti-flagellin (Fla) antibody response and isotype in the sera and intestinal washings of sheep, immunized with live aromatic-dependent (aro-) Salmonella typhimurium strain CS332 by the intramuscular (live i.m.) or oral (live oral) route or acetone-killed virulent S. typhimurium by the intramuscular route (killed i.m.), were determined at various intervals post-immunization. The serum or intestinal anti-lipopolysaccharide (LPS) or anti-flagellin (Fla) antibody titres of immunized sheep, regardless of the route of immunization, were significantly greater (P < 0.01) than those of non-immune control sheep. Although significant differences between the serum anti-LPS or anti-Fla antibody titres of sheep in various immunization regimes were observed, they were not consistent for different periods post-immunization. The predominant isotype contributing to serum anti-LPS antibody activity was IgM whereas the serum antiflagellar antibody activity was confined to IgM, IgG1 and IgG2. In either case, the contribution of the IgA antibody isotype was minimal. Antibody activity in the intestinal washings of immunized sheep, regardless of the route of immunization was significantly greater (P < 0.01) than that in non-immune control sheep. However, the titres in sheep immunized with the live S. typhimurium vaccines were significantly greater than those immunized with the killed vaccine. The major anti-LPS or anti-flagellin antibody isotype in the intestinal washings of sheep in the live i.m. or live oral groups was IgM at day 7 post-immunization followed by IgG1 and IgG2 at days 14 and 21 post-immunization, with only a minimal contribution by the IgA antibody isotype. On the other hand, the major antibody isotype in the intestinal washings of sheep immunized with the killed S. typhimurium was IgG1.
在研发出一种能够抑制肠道蛋白水解活性的合适制剂后，通过肌肉注射（活苗肌肉注射）或口服（活苗口服）途径用芳香族依赖型（aro-）鼠伤寒沙门氏菌CS332活菌株免疫绵羊，或通过肌肉注射途径用丙酮灭活的强毒鼠伤寒沙门氏菌（灭活苗肌肉注射）免疫绵羊，在免疫后的不同时间间隔测定绵羊血清和肠道冲洗液中的总抗脂多糖（LPS）和抗鞭毛蛋白（Fla）抗体反应及抗体亚型。无论免疫途径如何，免疫绵羊的血清或肠道抗脂多糖（LPS）或抗鞭毛蛋白（Fla）抗体滴度均显著高于（P < 0.01）非免疫对照绵羊。虽然观察到不同免疫方案中绵羊血清抗LPS或抗Fla抗体滴度存在显著差异，但在免疫后的不同时期并不一致。对血清抗LPS抗体活性起主要作用的抗体亚型是IgM，而血清抗鞭毛抗体活性则局限于IgM、IgG1和IgG2。在这两种情况下，IgA抗体亚型的贡献最小。无论免疫途径如何，免疫绵羊肠道冲洗液中的抗体活性均显著高于（P < 0.01）非免疫对照绵羊。然而，用鼠伤寒沙门氏菌活疫苗免疫的绵羊的抗体滴度显著高于用灭活疫苗免疫的绵羊。在免疫后第7天，活苗肌肉注射或活苗口服组绵羊肠道冲洗液中主要的抗LPS或抗鞭毛蛋白抗体亚型是IgM，随后在免疫后第14天和21天是IgG1和IgG2，IgA抗体亚型的贡献极小。另一方面，用灭活鼠伤寒沙门氏菌免疫的绵羊肠道冲洗液中的主要抗体亚型是IgG1。
