van Griethuysen Arjanne, van Loo Inge, van Belkum Alex, Vandenbroucke-Grauls Christina, Wannet Wim, van Keulen Peter, Kluytmans Jan
Department of Medical Microbiology and Medical Immunology, Internal Post Number 1630, Hospital Rijnstate Arnhem, P.O. Box 9025, 6800 EG Arnhem, The Netherlands.
J Clin Microbiol. 2005 Mar;43(3):1361-5. doi: 10.1128/JCM.43.3.1361-1365.2005.
The mecA gene was lost in 36 (14.4%) of 250 methicillin-resistant Staphylococcus aureus isolates after 2 years of storage at -80 degrees C with the Microbank system (Pro-lab Diagnostics, Austin, Tex.). Further analysis of 35 of these isolates confirmed loss of the mecA gene in 32 isolates. This finding has important implications for the management of strain collections.
在使用Microbank系统(Pro-lab Diagnostics,奥斯汀,德克萨斯州)于-80摄氏度储存2年后,250株耐甲氧西林金黄色葡萄球菌分离株中有36株(14.4%)的mecA基因丢失。对其中35株分离株的进一步分析证实,32株分离株中mecA基因丢失。这一发现对菌株保藏管理具有重要意义。