Cho Soung-Hun, Lee Byung-Doo, An Haejung, Eun Jong-Bang
Department of Food Science and Technology and Institute of Biotechnology, Chonnam National University, 300 Yongbong-dong Buk-gu, Gwangju 500-757, South Korea.
Peptides. 2005 Apr;26(4):581-7. doi: 10.1016/j.peptides.2004.11.011. Epub 2005 Jan 11.
An antimicrobial peptide was purified from fermented skate skin extract using the solid-phase extraction and separation on HPLC reversed-phase chromatography. Amino acid sequence of the purified peptide (Peak A) having an antimicrobial activity revealed the presence of many cationic residues of the total 28 amino acids. Its molecular mass was found to be 3059 Da. This result was in excellent agreement with the theoretical molecular mass calculated from the amino acid sequence. The synthetic kenojeinin I had inhibitory effects on B. subtilis (MIC, 12 microg/ml), E. coli (28 microg/ml), and S. cerevisiae (12 microg/ml). These results indicate that fermented skate skin is potentially antimicrobial.
采用固相萃取和高效液相色谱反相色谱法从发酵鳐鱼皮提取物中纯化出一种抗菌肽。具有抗菌活性的纯化肽（峰A）的氨基酸序列显示，在总共28个氨基酸中存在许多阳离子残基。其分子量为3059 Da。该结果与根据氨基酸序列计算出的理论分子量非常吻合。合成的kenojeinin I对枯草芽孢杆菌（最小抑菌浓度，12微克/毫升）、大肠杆菌（28微克/毫升）和酿酒酵母（12微克/毫升）具有抑制作用。这些结果表明发酵鳐鱼皮具有潜在的抗菌作用。
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