Institute of Military Veterinary Science, Academy of Military Medical Science, No. 666, Liu'ying West Road, Changchun, Jilin 130062, China.
Int J Antimicrob Agents. 2011 Dec;38(6):510-5. doi: 10.1016/j.ijantimicag.2011.07.012. Epub 2011 Oct 19.
A new antimicrobial peptide (AMP) named PGLa-H has been isolated from the skin of the African clawed frog (Xenopus laevis) using gel filtration and reverse-phase high-performance liquid chromatography (RP-HPLC). Its amino acid sequence was determined as KIAKVALKAL by Edman degradation, with a molecular weight of 1053.727 Da as analysed by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/TOF-MS). No similar AMP was found by BLAST search. Purified PGLa-H demonstrated antimicrobial ability against the reference bacteria Escherichia coli ATCC 25922 [minimum inhibitory concentration (MIC)=23.6 μg/mL], Staphylococcus aureus ATCC 25923 (MIC=8.7 μg/mL) and Bacillus subtilis (MIC=14.4μg/mL) and was active against multidrug-resistant meticillin-resistant S. aureus (MRSA) (MIC=67.8 μg/mL). The antimicrobial mechanism for this new peptide was further investigated by transmission electron microscopy. PGLa-H killed cells by destroying the cell membrane.
一种新的抗菌肽（AMP）已从非洲爪蟾（Xenopus laevis）的皮肤中分离出来，方法是使用凝胶过滤和反相高效液相色谱（RP-HPLC）。通过 Edman 降解确定其氨基酸序列为 KIAKVALKAL，通过基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF/TOF-MS）分析分子量为 1053.727 Da。BLAST 搜索未发现类似的 AMP。纯化的 PGLa-H 对参考细菌大肠杆菌 ATCC 25922[最小抑菌浓度（MIC）=23.6μg/mL]、金黄色葡萄球菌 ATCC 25923（MIC=8.7μg/mL）和枯草芽孢杆菌（MIC=14.4μg/mL）具有抗菌能力，对多药耐药性耐甲氧西林金黄色葡萄球菌（MRSA）（MIC=67.8μg/mL）也具有活性。通过透射电子显微镜进一步研究了这种新肽的抗菌机制。PGLa-H 通过破坏细胞膜杀死细胞。
