筛选印度尼西亚药用植物对RAW264.7细胞一氧化氮产生的抑制活性和抗氧化活性。

Screening of Indonesian medicinal plants for inhibitor activity on nitric oxide production of RAW264.7 cells and antioxidant activity.

作者信息

Choi Eun-Mi, Hwang Jae-Kwan

机构信息

Department of Biotechnology and Bioproducts Research Center, Yonsei University, Shinchon-dong 134, Seodaemun-gu, Seoul, 120-749, Korea.

出版信息

Fitoterapia. 2005 Mar;76(2):194-203. doi: 10.1016/j.fitote.2004.11.010.

DOI:10.1016/j.fitote.2004.11.010

PMID:15752630

Abstract

Traditional Indonesian medicinal plants were screened for their inhibitory effects on the nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and for the antioxidant activity through the evaluation of free radical scavenging effect and reducing power. The results of screening indicated that 50 methanolic extracts inhibited (>50%) lipopolysaccharides (LPS)-induced NO release from RAW264.7 cells at 50 microg/ml, with 18 having greater than 100% inhibition. At 200 microg/ml, 61 methanol extracts exhibited inhibitory activity (>50%), with 45 showing greater than 100% inhibition. In addition, the free radical scavenging effects of 6 methanolic extracts were found to be more than 50% for extract concentration of 0.5 mug/ml. The results indicate that the extracts contain active compounds that inhibit NO release and scavenge free radical.

摘要

对传统的印度尼西亚药用植物进行了筛选，以评估其对脂多糖（LPS）刺激的RAW264.7巨噬细胞中一氧化氮（NO）产生的抑制作用，并通过评估自由基清除作用和还原能力来检测其抗氧化活性。筛选结果表明，50种甲醇提取物在50微克/毫升时可抑制（>50%）脂多糖（LPS）诱导的RAW264.7细胞释放NO，其中18种的抑制率大于100%。在200微克/毫升时，61种甲醇提取物表现出抑制活性（>50%），45种的抑制率大于100%。此外，发现6种甲醇提取物在提取物浓度为0.5微克/毫升时的自由基清除作用超过50%。结果表明，这些提取物含有抑制NO释放和清除自由基的活性化合物。