Wiedmann-Al-Ahmad M, Gutwald R, Gellrich N-C, Hübner U, Schmelzeisen R
Klinik und Poliklinik für Mund-, Kiefer- und Gesichtschirurgie, Albert-Ludwigs-Universität Freiburg, Hugstetterstrasse 55, D-79106, Freiburg, Germany.
J Mater Sci Mater Med. 2005 Jan;16(1):57-66. doi: 10.1007/s10856-005-6447-z.
In this study we cultured human osteoblast-like cells on 16 different biomaterials to find an optimal biomaterial for subsequent use in reconstructive surgery. The tested biomaterials can be divided into five groups: collagen-based membranes of bovine, equine or calf origin, tricalcium phosphate based membranes (alpha and beta), hyaluronic acid based, anorganic bovine bone and anorganic silicone-based membranes. Cell proliferation and cell colonization (Environmental Scanning Electron Microscope, ESEM) analysis were performed. The results of the study demonstrated that four of the examined biomaterial/cell constructs showed a very good proliferation rate and cell density: No. 3 (Tissue Vlies), No. 7 (Sepra film), No. 16 (Biobrane) and No. 17 (Biomend). No favourable group of biomaterials was noticeable. Moreover, the results indicate that these four biomaterials as a part of bone constructs are the best tools for engineering new bone tissue. In contrast, biomaterials No. 19a (Bio-Oss) and 19b (Bio-Oss Collagen) showed the lowest proliferation rates. The result of No. 19b was improved by treatment in the perfusion chamber for 48 h as well as by additional use of vacuum. The present study is an important base for further analysis of biomaterials and consequently for the development of tissue engineering.
在本研究中，我们将人成骨样细胞培养在16种不同的生物材料上，以寻找一种最佳生物材料，供后续用于重建手术。所测试的生物材料可分为五组：牛、马或小牛来源的胶原基膜、磷酸三钙基膜（α型和β型）、透明质酸基膜、无机牛骨和无机硅基膜。进行了细胞增殖和细胞定植（环境扫描电子显微镜，ESEM）分析。研究结果表明，所检测的四种生物材料/细胞构建体显示出非常好的增殖率和细胞密度：3号（组织衬布）、7号（Sepra膜）、16号（生物膜）和17号（生物补片）。未发现有优势的生物材料组。此外，结果表明，这四种生物材料作为骨构建体的一部分，是工程化新骨组织的最佳工具。相比之下，19a号（Bio-Oss）和19b号（Bio-Oss胶原）生物材料的增殖率最低。19b号的结果通过在灌注室中处理48小时以及额外使用真空得到了改善。本研究是进一步分析生物材料以及进而开发组织工程的重要基础。
