Ayobian-Markazi Nader, Fourootan T, Kharazifar M J
Department of Periodontics, Dental Branch, Islamic Azad University, Tehran, Iran.
Dent Res J (Isfahan). 2012 Jan;9(1):86-92. doi: 10.4103/1735-3327.92959.
Many studies have shown favorable results following the use of different bone graft materials. The aim of the present study was to evaluate the biocompatibility of four different bone graft materials regarding cell viability and morphology of Human osteoblast-like cells (SaOS-2) in vitro.
The effects of Bio-Oss(®), Tutodent(®), Osteon(®), and Cerasorb(®) were studied on the human osteoblast-like cell line to evaluate various parameters. Human osteoblast-like cells were seeded onto the mentioned bone substitute materials (BSMs). Cell differentiation; cell viability and alkaline phosphatase (ALP) activity of the seeded cells were evaluated by means of scanning electron microscopy, cell viability test and phase contrast microscopy Analysis of variance (ANOVA). Tamhane's post-hoc, Kruskal-Wallis Test, and Dunn's Test were used. The results were considered to be statistically significant at P<0.05.
The control group (SaOS-2 cells which were incubated in Dulbecco Modified Eagle Medium without any kind of bone graft materials) had the highest level of cell viability (P<0.001), followed by Tutodent(®), Osteon(®), Cerasorb(®), and Bio-Oss(®). There was no significant difference in MTT assay results between Tutodent(®) and the control group (P=0.032). All tested bone graft materials showed significantly higher ALP activity than the control (P<0.001). The Tutodent(®) group showed the best cell growth among all experimental groups, followed by the Osteon(®) group. The former had a higher spindle-like morphology with good attachment to the surface. Cells cultivated on the surfaces of the Cerasorb(®) and Bio-Oss(®) granules had more round morphologies.
This in vitro study demonstrated that all tested BSMs can provide good cell differentiation but a lower rate of proliferation.
许多研究表明，使用不同的骨移植材料后会有良好的效果。本研究的目的是在体外评估四种不同骨移植材料对人成骨样细胞（SaOS-2）的细胞活力和形态的生物相容性。
研究了Bio-Oss®、Tutodent®、Osteon®和Cerasorb®对人成骨样细胞系的影响，以评估各种参数。将人成骨样细胞接种到上述骨替代材料（BSMs）上。通过扫描电子显微镜、细胞活力测试和相差显微镜对接种细胞的细胞分化、细胞活力和碱性磷酸酶（ALP）活性进行评估。采用方差分析（ANOVA）、Tamhanes事后检验、Kruskal-Wallis检验和Dunn检验。结果在P<0.05时被认为具有统计学意义。
对照组（在不含任何骨移植材料的杜氏改良 Eagle 培养基中培养的 SaOS-2 细胞）具有最高的细胞活力水平（P<0.001），其次是 Tutodent®、Osteon®、Cerasorb®和 Bio-Oss®。Tutodent®与对照组之间的MTT检测结果无显著差异（P=0.032）。所有测试的骨移植材料均显示出比对照组显著更高的ALP活性（P<0.001）。Tutodent®组在所有实验组中显示出最佳的细胞生长，其次是 Osteon®组。前者具有更高的纺锤状形态，与表面的附着良好。在Cerasorb®和Bio-Oss®颗粒表面培养的细胞具有更圆的形态。
这项体外研究表明，所有测试的BSMs都能提供良好的细胞分化，但增殖率较低。
