原发性肝癌中p57(kip2)的表达及其与临床病理、增殖细胞核抗原和p53的关系
Expression of p57(kip2) and its relationship with clinicopathology, PCNA and p53 in primary hepatocellular carcinoma.
作者信息
Nan Ke-Jun, Guo Hui, Ruan Zhi-Ping, Jing Zhao, Liu Shaan-Xi
机构信息
Department of Oncology, First Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.
出版信息
World J Gastroenterol. 2005 Feb 28;11(8):1237-40. doi: 10.3748/wjg.v11.i8.1237.
AIM
To investigate the expression of p57(kip2) and its relationship with clinicopathology, PCNA and p53 in primary hepatocellular carcinoma (HCC).
METHODS
Expression of p57(kip2), PCNA and p53 in tumor tissues from 32 patients with HCC and 10 liver tissues of normal persons was detected with Elivision immunohistochemical technique.
RESULTS
The p57(kip2) protein positive-expression rate in HCC was 56.25%, lower than that in normal tissues (100%, P<0.05). The reduced expression of p57(kip2) protein correlated significantly with moderate or low differentiation of tumor cells (P = 0.007 <0.05), high clinical stage (P = 0.041 <0.05) and poor prognosis (P = 0.036 <0.05), but did not correlate significantly with metastasis, tumor size, level of AFP and age (P>0.05). The PCNA positive-expression rate was 56.25%, which was correlated significantly with the expression of p57(kip2) (P = 0.025<0.05). The p53 positive-expression rate was 46.88%, which was not correlated significantly with the expression of p57(kip2) (P>0.05).
CONCLUSION
There is a marked loss or absence of p57(kip2) expression and high expression of PCNA in HCC, which are involved in carcinogenesis and development of HCC. The p57(kip2) and p53 may induce apoptosis via different mechanisms.
目的
探讨p57（kip2）在原发性肝细胞癌（HCC）中的表达及其与临床病理、增殖细胞核抗原（PCNA）和p53的关系。
方法
采用Elivision免疫组化技术检测32例HCC患者肿瘤组织及10例正常人肝组织中p57（kip2）、PCNA和p53的表达。
结果
HCC中p57（kip2）蛋白阳性表达率为56.25%，低于正常组织（100%，P<0.05）。p57（kip2）蛋白表达降低与肿瘤细胞中、低分化（P = 0.007<0.05）、临床分期高（P = 0.041<0.05）及预后差（P = 0.036<0.05）显著相关，但与转移、肿瘤大小、甲胎蛋白水平及年龄无显著相关性（P>0.05）。PCNA阳性表达率为56.25%，与p57（kip2）表达显著相关（P = 0.025<0.05）。p53阳性表达率为46.88%，与p57（kip2）表达无显著相关性（P>0.05）。
结论
HCC中存在明显的p57（kip2）表达缺失或降低及PCNA高表达，它们参与了HCC的发生发展。p57（kip2）和p53可能通过不同机制诱导细胞凋亡。
Zotero 插件