Yue Hui, Zhang Ning, Feng Xin-Li, Ma Shu-Ren, Song Fu-Lin, Yang Ming, Tang Yi-Hai
Center of Gastrointestinal Endoscopy, General Hospital of Shenyang Military Command, Shenyang, Liaoning, 110016, PR China.
Ai Zheng. 2003 Jul;22(7):705-9.
BACKGROUND & OBJECTIVE: The abnormality of mammalian cell cycle regulation is an important cause of cell over-proliferation and oncogenesis. There were few reports about the relationship between p57(kip2) protein as negative factor of cell cycle regulation and pancreatic cancer. This article aims to investigate the effects of p57(kip2), cyclin E and proliferating cell nuclear antigen (PCNA) protein on the occurrence and progression of pancreatic cancer.
Expression of p57(kip2), cyclin E, and PCNA in tumor tissue and adjacent tissue from 32 patients with pancreatic cancer were detected using SP immunohistochemical technique.
The positive-expression rate of p57(kip2) protein in tumor tissue of pancreatic cancer was 46.9%, which was lower than that in adjacent pancreatic tissue (75.0%) (Chi(2)=5.317, P< 0.05); p57(kip2) protein positive-expression was remarkably correlated with tumor cell differentiation (P< 0.05), but was not correlated with lymph node metastasis (P >0.05). The positive-expression rate of cyclin E in tumor tissues was 68.8%, which was higher than that in adjacent pancreatic tissue (43.8%) (Chi(2)=4.063,P< 0.05); Cyclin E positive-expression was remarkably correlated with tumor cell differentiation and lymph node metastasis (P< 0.05). The positive-expression rate of PCNA protein in tumor tissues was 71.9%, which was higher than that in adjacent pancreatic tissue (43.8%) (Chi(2)=5.189,P< 0.05); PCNA positive- expression was remarkably correlated with tumor cell differentiation and lymph node metastasis(P< 0.05).
The decreased expression of p57(kip2) protein and over-expression of cyclin E and PCNA proteins may significantly related to genesis and progress of pancreatic cancer.
哺乳动物细胞周期调控异常是细胞过度增殖和肿瘤发生的重要原因。关于作为细胞周期调控负性因子的p57(kip2)蛋白与胰腺癌之间的关系，此前鲜有报道。本文旨在研究p57(kip2)、细胞周期蛋白E（Cyclin E）和增殖细胞核抗原（PCNA）蛋白对胰腺癌发生发展的影响。
采用SP免疫组化技术检测32例胰腺癌患者肿瘤组织及癌旁组织中p57(kip2)、Cyclin E和PCNA的表达情况。
胰腺癌肿瘤组织中p57(kip2)蛋白阳性表达率为46.9%，低于癌旁胰腺组织（75.0%）（χ2=5.317，P<0.05）；p57(kip2)蛋白阳性表达与肿瘤细胞分化显著相关（P<0.05），但与淋巴结转移无关（P>0.05）。肿瘤组织中Cyclin E阳性表达率为68.8%，高于癌旁胰腺组织（43.8%）（χ2=4.063，P<0.05）；Cyclin E阳性表达与肿瘤细胞分化及淋巴结转移显著相关（P<0.05）。肿瘤组织中PCNA蛋白阳性表达率为71.9%，高于癌旁胰腺组织（43.8%）（χ2=5.189，P<0.05）；PCNA阳性表达与肿瘤细胞分化及淋巴结转移显著相关（P<0.05）。
p57(kip2)蛋白表达降低以及Cyclin E和PCNA蛋白过度表达可能与胰腺癌的发生发展密切相关。
