p57kip2、Rb蛋白和增殖细胞核抗原在人胰腺癌中的表达及其与临床病理的关系。
Expression of p57kip2, Rb protein and PCNA and their relationships with clinicopathology in human pancreatic cancer.
作者信息
Yue Hui, Na Yan-Li, Feng Xin-Li, Ma Shu-Ren, Song Fu-Lin, Yang Bo
机构信息
Department of Gastroenterology, General Hospital of Shenyang Military Region, Shenyang 110016, Liaoning Province, China.
出版信息
World J Gastroenterol. 2003 Feb;9(2):377-80. doi: 10.3748/wjg.v9.i2.377.
AIM
To investigate the effects of inhibiting factor of cell cycle regulation p57(kip2), retinoblastinoma protein (Rb protein) and proliferating cell nuclear antigen (PCNA) in the genesis and progression of human pancreatic cancer.
METHODS
The expression of p57(kip2), Rb protein and PCNA in tumor tissues and adjacent tissues of 32 patients with pancreatic cancer was detected with SP immunohistochemical technique.
RESULTS
p57(kip2) protein positive-expression rate in tumor tissues of pancreatic cancer was 46.9 %, which was lower than that in adjacent pancreatic tissues (75.0 %) (chi(2)=5.317, P<0.05), p57(kip2) protein positive-expression correlated significantly with tumor cell differentiation (well-differentiation versus moderate or low-differentiation, P<0.05) but did not correlate significantly with lymph node metastasis (lymph node metastasis versus non-lymph node metastasis, P>0.05); Rb gene protein positive-expression rate in tumor tissues was 50.0 %, which was also lower than that in adjacent pancreatic tissues (78.1 %) (chi(2)=5.497, P<0.05); PCNA positive-expression rate was 71.9 %, being higher than that in adjacent pancreatic tissues (43.8 %) (chi(2)=5.189, P<0.05), PCNA positive-expression also correlated significantly with tumor cell differentiation and lymph node metastasis (well-differentiation versus moderate or low- differentiation, lymph node metastasis versus non-lymph node metastasis, P<0.05). Rb protein positive-expression rate in the tumor tissues of p57(kip2) protein positive-expression group was 53.3 %; and Rb protein positive-expression rate in the tumor tissues of p57(kip2) protein negative-expression group was 47.1 %. There was no significant relationship between the two groups (r=0.16507, P>0.05).
CONCLUSION
The decreased expression of p57(kip2), Rb protein or over-expression of PCNA protein might contribute to the genesis or progression of pancreatic cancer, p57(kip2), Rb protein and PCNA may play an important role in genesis and progression of pancreatic cancer.
目的
探讨细胞周期调控抑制因子p57（kip2）、视网膜母细胞瘤蛋白（Rb蛋白）和增殖细胞核抗原（PCNA）在人胰腺癌发生发展中的作用。
方法
采用SP免疫组化技术检测32例胰腺癌患者肿瘤组织及癌旁组织中p57（kip2）、Rb蛋白和PCNA的表达。
结果
胰腺癌肿瘤组织中p57（kip2）蛋白阳性表达率为46.9%，低于癌旁胰腺组织（75.0%）（χ2=5.317，P<0.05），p57（kip2）蛋白阳性表达与肿瘤细胞分化显著相关（高分化与中低分化比较，P<0.05），但与淋巴结转移无显著相关性（有淋巴结转移与无淋巴结转移比较，P>0.05）；肿瘤组织中Rb基因蛋白阳性表达率为50.0%，也低于癌旁胰腺组织（78.1%）（χ2=5.497，P<0.05）；PCNA阳性表达率为71.9%，高于癌旁胰腺组织（43.8%）（χ2=5.189，P<0.05），PCNA阳性表达也与肿瘤细胞分化及淋巴结转移显著相关（高分化与中低分化比较，有淋巴结转移与无淋巴结转移比较，P<0.05）。p57（kip2）蛋白阳性表达组肿瘤组织中Rb蛋白阳性表达率为53.3%；p57（kip2）蛋白阴性表达组肿瘤组织中Rb蛋白阳性表达率为为47.1%。两组间无显著相关性（r=0.16507，P>0.05）。
结论
p57（kip2）、Rb蛋白表达降低或PCNA蛋白过表达可能与胰腺癌的发生发展有关，p57（kip2）、Rb蛋白和PCNA可能在胰腺癌的发生发展中起重要作用。
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