Zevenhuizen L P
Laboratory of Microbiology, Agricultural University, Wageningen, The Netherlands.
Antonie Van Leeuwenhoek. 1992 Jan;61(1):61-8. doi: 10.1007/BF00572124.
Cells of Arthrobacter globiformis grown in carbohydrate-rich media were found to contain large quantities of low-Mr carbohydrates (800 micrograms/mg protein) and only small amounts of amino acids, in addition to high amounts of glycogen (2 mg/mg protein). At increasing osmotic values of the medium, low-Mr carbohydrate levels increased to 1300 micrograms/mg protein. Low-Mr pools were extracted from the cells with hot 75% ethanol, and subjected to thin layer, gel and gas-liquid chromatography. They turned out to consist mainly of alpha,alpha-trehalose. Levels of trehalose in Arthrobacter cells have the tendency to remain constant, both during nutrient exhaustion (resulting in glycogen consumption), and on addition of excess of carbon source to the medium (resulting in an increased glycogen content of the cells). The stress-tolerant properties of Arthrobacter (resistance to nutrient starvation, desiccation and high salt concentration) are discussed with respect to the high glycogen and trehalose contents of the cells.
研究发现，在富含碳水化合物的培养基中生长的球形节杆菌细胞，除了含有大量糖原（2毫克/毫克蛋白质）外，还含有大量低分子量碳水化合物（800微克/毫克蛋白质），而氨基酸含量很少。随着培养基渗透压的增加，低分子量碳水化合物水平增加到1300微克/毫克蛋白质。用热的75%乙醇从细胞中提取低分子量组分，并进行薄层色谱、凝胶色谱和气液色谱分析。结果表明，它们主要由α,α-海藻糖组成。在营养耗尽（导致糖原消耗）期间以及向培养基中添加过量碳源（导致细胞糖原含量增加）时，球形节杆菌细胞中海藻糖水平都有保持恒定的趋势。针对细胞中高糖原和海藻糖含量，讨论了球形节杆菌的抗逆特性（对营养饥饿、干燥和高盐浓度的抗性）。
