巴西中部联邦区献血者中的丙型肝炎病毒基因型

Hepatitis C virus genotypes in blood donors from the Federal District, Central Brazil.

作者信息

Amorim Regina M S, Oliveira Claudiner P, Wyant Patrícia S, Cerqueira Daniela M, Câmara Geni N L, Flores Luciano S, Martins Regina M B, Martins Cláudia R F

机构信息

Departamento de Biologia Celular, Instituto de Biologia, Universidade de Brasília, ICC Sul, 70919-900 Brasília, DF, Brazil.

出版信息

Mem Inst Oswaldo Cruz. 2004 Dec;99(8):895-7. doi: 10.1590/s0074-02762004000800019. Epub 2005 Mar 4.

DOI:10.1590/s0074-02762004000800019

PMID:15761609

Abstract

The objective of this study was to characterize hepatitis C virus (HCV) genotypes in blood donors from the Federal District, Central Brazil, and to compare HCV screening by serological assays and reverse transcriptase polymerase chain reaction (RT-PCR). Plasma samples from 57 individuals with reactive or indeterminate results in serological anti-HCV screening assays (ELISA or EIA) were tested for HCV RNA by RT-PCR. The results from a confirmatory LIA serological assay were also evaluated. The 5' non-coding region of the HCV genome was amplified from 41 PCR positive samples (71.9%), which were further characterized by nucleotide sequencing analysis. Of these, 60.9% were of HCV genotype 1 and 39.1% of genotype 3.

摘要

本研究的目的是对巴西中部联邦区献血者的丙型肝炎病毒（HCV）基因型进行特征分析，并比较血清学检测和逆转录聚合酶链反应（RT-PCR）检测HCV的情况。对57名在血清学抗HCV筛查检测（ELISA或EIA）中结果呈阳性或不确定的个体的血浆样本进行RT-PCR检测HCV RNA。还评估了确证性LIA血清学检测的结果。从41份PCR阳性样本（71.9%）中扩增出HCV基因组的5'非编码区，并通过核苷酸测序分析进一步对其进行特征分析。其中，60.9%为HCV 1型，39.1%为3型。