Mulheron G W, Schomberg D W
Department of Obstetrics and Gynecology, Duke University Medical Center, Durham, North Carolina 27710.
Biol Reprod. 1992 Apr;46(4):546-50. doi: 10.1095/biolreprod46.4.546.
Recent studies indicate that reproductive hormones may stimulate target cell growth via the actions of growth factors. In this study, reverse transcription-polymerase chain reaction analysis was used to assess the effects of in vivo diethylstilbestrol (DES) treatment on granulosa cell (GC) and thecal-interstitial cell (TIC) transforming growth factor (TGF)-beta 2 mRNA expression. In response to DES, GC expression of TGF-beta 2 mRNA was stimulated approximately 6.8-fold. By contrast, DES did not significantly alter TIC expression of TGF-beta 2 mRNA. Data were normalized relative to glyceraldehyde-3-phosphate dehydrogenase mRNA levels, which were unaffected by DES. These results extend our earlier findings, which demonstrated that GC and TIC expression of TGF-beta 2 mRNA is regulated by gonadotropins in vitro, to include steroidal regulation in vivo of intraovarian growth factor expression. Collectively, the present study and previous results support the following concepts: 1) intraovarian TGF-beta 2 mRNA expression is regulated by both gonadotropins and steroids, and 2) GC and TIC expression of TGF-beta 2 mRNA are differentially regulated.
近期研究表明，生殖激素可能通过生长因子的作用来刺激靶细胞生长。在本研究中，采用逆转录-聚合酶链反应分析来评估体内己烯雌酚（DES）处理对颗粒细胞（GC）和卵泡膜间质细胞（TIC）转化生长因子（TGF）-β2 mRNA表达的影响。作为对DES的反应，GC中TGF-β2 mRNA的表达受到约6.8倍的刺激。相比之下，DES并未显著改变TIC中TGF-β2 mRNA的表达。数据相对于甘油醛-3-磷酸脱氢酶mRNA水平进行了标准化，后者不受DES影响。这些结果扩展了我们早期的发现，即早期发现表明GC和TIC中TGF-β2 mRNA的表达在体外受促性腺激素调节，现在则扩展到包括体内卵巢内生长因子表达的甾体调节。总体而言，本研究和先前结果支持以下概念：1）卵巢内TGF-β2 mRNA的表达受促性腺激素和甾体的共同调节；2）GC和TIC中TGF-β2 mRNA的表达受到不同的调节。
