Law A S, Burt D W, Armstrong D G
Division of Molecular Biology, Roslin Institute (Edinburgh), United Kingdom.
Gen Comp Endocrinol. 1995 Jun;98(3):227-33. doi: 10.1006/gcen.1995.1064.
RNA was isolated from chicken thecal tissue from the largest (F1), third largest (F3), and fifth largest (F5) preovulatory follicles, from small yolky follicles, and from granulosa tissue from F1 follicles. Transforming growth factor-beta (TGF-beta) gene expression was measured using reverse transcription polymerase chain reaction. Thecal cells from all follicle sizes expressed all three isoforms of TGF-beta. TGF-beta 1 mRNA was detected in granulosa cells at levels comparable to those seen in thecal cells. However, TGF-beta 2 and TGF-beta 3 mRNA was expressed at significantly lower levels in granulosa than in thecal tissue. This is the first demonstration of TGF-beta gene expression in the ovary of a nonmammalian species. The similarities between mammalian and avian TGF-beta gene expression are remarkable, especially in light of the distinctive patterns of avian follicular development and the differing steroidogenic capacities of ovarian cell types of the two classes of vertebrates.
从鸡的最大（F1）、第三大（F3）和第五大（F5）排卵前卵泡的卵泡膜组织、小的有卵黄卵泡以及F1卵泡的颗粒细胞组织中分离RNA。使用逆转录聚合酶链反应测量转化生长因子-β（TGF-β）基因表达。所有卵泡大小的卵泡膜细胞均表达TGF-β的所有三种亚型。在颗粒细胞中检测到的TGF-β1 mRNA水平与在卵泡膜细胞中观察到的水平相当。然而，TGF-β2和TGF-β3 mRNA在颗粒细胞中的表达水平明显低于卵泡膜组织。这是首次在非哺乳动物物种的卵巢中证明TGF-β基因表达。哺乳动物和禽类TGF-β基因表达之间的相似性非常显著，特别是考虑到禽类卵泡发育的独特模式以及这两类脊椎动物卵巢细胞类型不同的类固醇生成能力。
