Jensen Lars Juhl, Steinmetz Lars M
European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
FEBS Lett. 2005 Mar 21;579(8):1802-7. doi: 10.1016/j.febslet.2005.02.006.
To understand a biological process it is clear that a single approach will not be sufficient, just like a single measurement on a protein--such as its expression level--does not describe protein function. Using reference sets of proteins as benchmarks different approaches can be scaled and integrated. Here, we demonstrate the power of data re-analysis and integration by applying it in a case study to data from deletion phenotype screens and mRNA expression profiling.
要理解一个生物学过程,显然单一方法是不够的,就如同对一种蛋白质进行单一测量——比如其表达水平——并不能描述蛋白质的功能一样。将蛋白质参考集用作基准,可以对不同方法进行量化和整合。在此,我们通过在一个案例研究中将其应用于缺失表型筛选和mRNA表达谱分析的数据,展示了数据重新分析和整合的强大功能。