Saudan Christophe, Desmarchelier Aurélien, Sottas Pierre-Edouard, Mangin Patrice, Saugy Martial
Laboratoire Suisse d'Analyse du Dopage, Institut Universitaire de Médecine Légale, Rue du Bugnon 21, 1005 Lausanne, Switzerland.
Steroids. 2005 Mar;70(3):179-83. doi: 10.1016/j.steroids.2004.12.007.
Pregnenolone (PREG) can potentially be abused by athletes to maintain an equilibration of the steroidal environment after sex steroids administrations. Five men volunteers orally ingested 50 mg PREG to determine optimal urinary markers for detection of this steroid. Our findings show that ingestion of PREG has no significant effects on the testosterone/epitestosterone (T/E) and testosterone/luteinizing hormone (T/LH) ratios, whereas variable changes on the carbon isotopic values of three T metabolites: androsterone, etiocholanolone, 5beta-androstane-3alpha,17beta-diol (5beta-androstanediol) together with 16(5alpha)-androsten-3alpha-ol (androstenol) and 5beta-pregnane-3alpha,20alpha-diol (pregnanediol) have been observed. The difference between the carbon isotopic values (delta13C-values) of androstenol and pregnanediol is potentially the most reliable marker of exogenous PREG administration in males. For all subjects, the differences differ by 3.0 per thousand or more over a period of about 10 h and for both of them the detection window for positivity is extended over 40 h.
孕烯醇酮（PREG）可能会被运动员滥用，以在使用性类固醇后维持甾体环境的平衡。五名男性志愿者口服了50毫克PREG，以确定检测这种类固醇的最佳尿液标志物。我们的研究结果表明，摄入PREG对睾酮/表睾酮（T/E）和睾酮/促黄体生成素（T/LH）比值没有显著影响，而三种睾酮代谢物：雄酮、表雄酮、5β-雄甾烷-3α,17β-二醇（5β-雄甾二醇）以及16(5α)-雄甾烯-3α-醇（雄烯醇）和5β-孕烷-3α,20α-二醇（孕二醇）的碳同位素值出现了可变变化。雄烯醇和孕二醇的碳同位素值（δ13C值）之间的差异可能是男性外源性PREG给药最可靠的标志物。对于所有受试者，在大约10小时的时间段内，两者的差异相差3.0‰或更多，并且两者的阳性检测窗口都延长至40小时以上。
