Kern Travis, Yang Yunzhi, Glover Renee, Ong Joo L
Department of Restorative Dentistry, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
Implant Dent. 2005 Mar;14(1):70-6. doi: 10.1097/01.id.0000154795.93155.ee.
The clinical success of dental implants is governed in part by surface properties of implants and their interactions with the surrounding tissues. The objective of this study was to investigate the effect of heat-treated titanium surfaces on protein adsorption and osteoblast precursor cell attachment in vitro. Passivated titanium samples used in this study were either non heat treated or heat treated at 750 degrees C for 90 minutes. It was observed that the contact angle on heat-treated titanium surfaces was statistically lower compared with the non-heat-treated titanium surfaces. The non-heat-treated titanium surface was also observed to be amorphous oxide, whereas heat treatment of titanium resulted in the conversion of amorphous oxide to crystalline anatase oxide. No significant difference in albumin and fibronectin adsorption was observed between the heat-treated and non-heat-treated titanium surfaces. In addition, no significant difference in initial cell attachment was observed between the two groups. It was concluded that heat treatment of titanium resulted in significantly more hydrophilic surfaces compared to non-heat-treated titanium surfaces. However, differences in oxide crystallinity and wettability were not observed to affect protein adsorption and initial osteoblast precursor cell attachment.
牙种植体的临床成功部分取决于种植体的表面特性及其与周围组织的相互作用。本研究的目的是在体外研究热处理钛表面对蛋白质吸附和成骨细胞前体细胞附着的影响。本研究中使用的钝化钛样品要么未经热处理，要么在750℃下热处理90分钟。观察到，与未热处理的钛表面相比，热处理钛表面的接触角在统计学上更低。还观察到未热处理的钛表面为非晶态氧化物，而钛的热处理导致非晶态氧化物转变为结晶锐钛矿氧化物。在热处理和未热处理的钛表面之间，白蛋白和纤连蛋白吸附没有显著差异。此外，两组之间在初始细胞附着方面没有观察到显著差异。得出的结论是，与未热处理的钛表面相比，钛的热处理导致表面亲水性显著增强。然而，未观察到氧化物结晶度和润湿性的差异会影响蛋白质吸附和初始成骨细胞前体细胞附着。
