培养的小鼠少突胶质细胞和星形胶质细胞中72千道尔顿热休克蛋白（HSP72）的异质性诱导。

Heterogeneous induction of 72-kDa heat shock protein (HSP72) in cultured mouse oligodendrocytes and astrocytes.

作者信息

Satoh J, Yamamura T, Kunishita T, Tabira T

机构信息

Division of Demyelinating Diseases and Aging, National Institute of Neuroscience, NCNP, Tokyo, Japan.

出版信息

Brain Res. 1992 Feb 21;573(1):37-43. doi: 10.1016/0006-8993(92)90111-l.

DOI:10.1016/0006-8993(92)90111-l

PMID:1576534

Abstract

The expression of 72-kDa heat shock protein (HSP72) in cultured mouse oligodendrocytes and astrocytes exposed to heat shock was investigated by double immunolabelling with anti-HSP72 monoclonal antibody (C92F3B-1) and antibodies against galactocerebroside (GalC) or glial fibrillary acidic protein (GFAP). After 3 h recovery from heat shock, an intermediate level of HSP72 immunolabelling was localized in the nucleolus and cytoplasm of astrocytes (less than 25%) and to a lesser extent in oligodendrocytes (less than 2%). After 8-48 h, HSP72 was expressed intensely in the cytoplasm and nuclear matrix of oligodendrocytes (20-40%), while weak/intermediate immunostaining was detectable in astrocytes (5-15%). The levels of HSP72 expressed in oligodendrocytes and astrocytes decreased around 72-120 h, but a few oligodendrocytes (4%) remained intensely immunolabelled. These results indicate that heat shock induces HSP72 in both oligodendrocytes and astrocytes. However, this response is heterogeneous.

摘要

通过使用抗HSP72单克隆抗体（C92F3B-1）以及抗半乳糖脑苷脂（GalC）或胶质纤维酸性蛋白（GFAP）的抗体进行双重免疫标记，研究了热休克条件下培养的小鼠少突胶质细胞和星形胶质细胞中72-kDa热休克蛋白（HSP72）的表达情况。热休克恢复3小时后，HSP72免疫标记的中间水平定位于星形胶质细胞的核仁和细胞质中（不到25%），在少突胶质细胞中的定位程度较低（不到2%）。8至48小时后，HSP72在少突胶质细胞的细胞质和核基质中强烈表达（20%至40%），而在星形胶质细胞中可检测到弱/中等免疫染色（5%至15%）。少突胶质细胞和星形胶质细胞中表达的HSP72水平在72至120小时左右下降，但仍有少数少突胶质细胞（4%）保持强烈的免疫标记。这些结果表明，热休克可在少突胶质细胞和星形胶质细胞中诱导HSP72。然而，这种反应是异质性的。