Watabe K, Osborne D, Kim S U
Department of Medicine, University of British Columbia, Vancouver, Canada.
J Neuropathol Exp Neurol. 1989 Sep;48(5):499-506. doi: 10.1097/00005072-198909000-00001.
The phagocytic activity of human glial cells was examined in primary cultures obtained from normal human brain obtained at autopsy. Highly enriched cultures of astrocytes and oligodendrocytes were established using an enzyme digestion-Percoll density gradient method. These cultures were exposed to medium containing carbon particles (indian ink) for one-24 hours. Under phase contrast and immunofluorescence microscopy, carbon particles were demonstrated in the cytoplasm and processes of both astrocytes and oligodendrocytes. Electron microscopic examination revealed carbon particles phagocytized and segregated in these cells. These findings may support a view that both astrocytes and oligodendrocytes participate in phagocytosis in certain neurological diseases.
在取自尸检正常人脑的原代培养物中检测了人类神经胶质细胞的吞噬活性。使用酶消化-派洛宁密度梯度法建立了高度富集的星形胶质细胞和少突胶质细胞培养物。将这些培养物暴露于含有碳颗粒（印度墨水）的培养基中1至24小时。在相差显微镜和免疫荧光显微镜下，在星形胶质细胞和少突胶质细胞的细胞质及突起中均发现了碳颗粒。电子显微镜检查显示这些细胞吞噬并隔离了碳颗粒。这些发现可能支持这样一种观点，即星形胶质细胞和少突胶质细胞在某些神经系统疾病中均参与吞噬作用。
