Huhn Carolin, Neusüss Christian, Pelzing Matthias, Pyell Ute, Mannhardt Joachim, Pütz Michael
Philipps-University Marburg, Analytical Chemistry, Marburg, Germany.
Electrophoresis. 2005 Apr;26(7-8):1389-97. doi: 10.1002/elps.200410163.
The simultaneous hyphenation of capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection and electrospray ionization-mass spectrometry (ESI-MS) as a novel combined detection system for CE is presented. beta-Carbolines were chosen as model analytes with a forensic background. Nonaqueous CE as well as conventional CE with an aqueous buffer system are compared concerning efficiency and obtainable detection limits. The distance between the optical detection window and the sprayer tip was minimized by placing the optical cell directly in front of the electrospray interface. Similar separation efficiencies for both detection modes could thus be obtained. No significant peak-broadening induced by the MS interface was observed. The high fluorescence quantum yield and the high proton affinity of the model analytes investigated resulted in limits of detection in the fg (nmol/L) range for both detection methods. The analysis of confiscated ayahuasca samples and ethanolic plant extracts revealed complementary selectivities for LIF and MS detection. Thus, it is possible to improve peak identification of the solutes investigated by the use of these two detection principles.
本文介绍了一种将毛细管电泳（CE）与激光诱导荧光（LIF）检测及电喷雾电离质谱（ESI-MS）同时联用的新型CE组合检测系统。选择具有法医背景的β-咔啉作为模型分析物。比较了非水CE以及使用水性缓冲体系的传统CE在效率和可获得的检测限方面的情况。通过将光学检测池直接置于电喷雾接口前方，使光学检测窗口与喷雾器尖端之间的距离最小化。因此，两种检测模式可获得相似的分离效率。未观察到MS接口引起的显著峰展宽。所研究的模型分析物具有高荧光量子产率和高质子亲和力，两种检测方法的检测限均在fg（nmol/L）范围内。对查获的死藤水样品和乙醇植物提取物的分析表明，LIF和MS检测具有互补的选择性。因此，利用这两种检测原理可以改善所研究溶质的峰识别。
