Reischwitz A, Stoppok E, Buchholz K
Sugar Institute, Institut für Technologie der Kohlenhydrate, Technical University of Braunschweig, Braunschweig, Germany.
Biodegradation. 1997;8(5):313-9. doi: 10.1023/a:1008203525476.
The anaerobic degradation of the polyesters poly-3-hydroxybutyrate (PHB) and poly-3-hydroxybutyrate-co-3-hydroxyvalerate (PHBV) was investigated with special regard to intermediate products, kinetics, and yields. During the degradation of PHBV acetate, propionate, n-butyrate, and n-valerate were detected. Additionally, 3-hydroxybutyrate and 3-hydroxyvalerate and four dimeric esters of these two molecules were identified by GC-MS measurements. Three different test systems for the anaerobic degradation of polyesters were studied. It was not possible to get reproducible results by means of the Anaerobic Sturm-test, a simple system based on carbon dioxide measurement. Secondly, a system based on the GC measurement of accumulated organic acids was investigated. A degradation of 90% in two days was calculated by a carbon balance. Best results were reached with the third test system based on the measurement of methane with a gas meter. A degradation of 99% was observed within 30 days.
对聚-3-羟基丁酸酯（PHB）和聚-3-羟基丁酸酯-共-3-羟基戊酸酯（PHBV）的厌氧降解进行了研究，特别关注中间产物、动力学和产率。在PHBV降解过程中，检测到了乙酸盐、丙酸盐、正丁酸盐和正戊酸盐。此外，通过气相色谱-质谱联用（GC-MS）测量鉴定出了3-羟基丁酸酯、3-羟基戊酸酯以及这两种分子的四种二聚酯。研究了三种不同的聚酯厌氧降解测试系统。通过基于二氧化碳测量的简单厌氧斯特姆测试系统无法获得可重复的结果。其次，研究了一种基于气相色谱测量积累有机酸的系统。通过碳平衡计算得出在两天内降解率为90%。基于用气体流量计测量甲烷的第三个测试系统取得了最佳结果。在30天内观察到降解率为99%。
