Cao Xiao-an, Chen Yong-heng, Lin Hua-min
Biology and Chemistry Engineering Institute, Guangzhou University, Guangzhou 510405, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2004 Apr;24(4):474-6.
The chromogenic reaction of 2-hydroxy-5-sulfobenzenediazoaminoazobenzene (HSDAA) with mercury was studied. In the presence of Triton X-100 and in a borax-buffer solution of pH 10.30, mercury(II) reacted with HSDAA to form a stable orange-red complex with molar radio of 1:2. The apparent molar absorptivity was 1.67 x 10(5) x L mol(-1) x cm(-1) with the maximum absorption wavelength of 519 nm. Beer's law was obeyed in the concentration range of 0-600 microg x L(-1) for Hg(II). The method was applied to the determination of trace amounts of mercury in water with satisfactory results.
研究了2-羟基-5-磺基苯重氮氨基偶氮苯（HSDAA）与汞的显色反应。在Triton X-100存在下，于pH 10.30的硼砂缓冲溶液中，汞（II）与HSDAA反应形成稳定的橙红色络合物，摩尔比为1:2。表观摩尔吸光系数为1.67×10⁵ L·mol⁻¹·cm⁻¹，最大吸收波长为519 nm。汞（II）在0 - 600 μg·L⁻¹浓度范围内遵守比尔定律。该方法用于测定水中痕量汞，结果令人满意。
