Department of Chemistry, Faculty of Science, King Abdulaziz University, P. O. Box 80203, Jeddah 21589, Saudi Arabia.
J Hazard Mater. 2010 Jun 15;178(1-3):287-92. doi: 10.1016/j.jhazmat.2010.01.075. Epub 2010 Jan 21.
A simple and low cost method was developed and validated for the determination of trace mercury (II) ions in dental-unit wastewater and fertilizer samples. The method was based upon the reaction of mercury (II) ions with the novel reagent 6-hydroxy-3-(2-oxoindolin-3-ylideneamino)-2-thioxo-2H-1,3-thiazin-4(3H)-one, the formed complex shows an absorption maximum at 505 nm (lambda(max)) in Britton-Robinson (B-R) buffer (pH 4-6).The corrected absorbance of the formed complex at lambda(max) was obtained employing beta-correction spectrophotometric method. Beer's-Lambert law and Ringbom's plots of the colored Hg-reagent complex were obeyed in the concentration range of 0.2-2.0 and 0.32-0.96 microg mL(-1) mercury (II) ions, respectively with a relative standard deviation in the range of 2.1+/-1.3%. The limits of detection (LOD) and quantification (LOQ) of the procedure were 0.026 and 0.086 microg mL(-1) Hg(2+), respectively. The proposed method was applied for the analysis of mercury (II) in dental-unit wastewater and fertilizer samples. The validation of the method was tested by comparison with the data obtained by the inductively coupled plasma-mass spectrometry (ICP-MS). The statistical treatment of data in terms of Student's t-tests and variance ratio f-tests has revealed no significance differences.
一种简单且低成本的方法被开发并验证用于测定牙科单位废水中痕量汞(II)离子和肥料样品中的汞(II)离子。该方法基于汞(II)离子与新型试剂 6-羟基-3-(2-氧代吲哚啉-3-亚基氨基)-2-硫代-2H-1,3-噻嗪-4(3H)-酮的反应，形成的配合物在 Britton-Robinson (B-R)缓冲液(pH 4-6)中在 505nm 处显示最大吸收(max)（lambda(max)）。在 lambda(max)处形成的配合物的校正吸光度采用β-校正分光光度法获得。Beer's-Lambert 定律和有色 Hg-试剂配合物的 Ringbom 图在 0.2-2.0 和 0.32-0.96μg mL(-1)汞(II)离子浓度范围内分别得到遵守，相对标准偏差在 2.1+/-1.3%范围内。该方法的检测限(LOD)和定量限(LOQ)分别为 0.026 和 0.086μg mL(-1) Hg(2+)。该方法用于分析牙科单位废水中的汞(II)和肥料样品。该方法的验证通过与电感耦合等离子体质谱法(ICP-MS)获得的数据进行比较来测试。数据的统计学处理在学生 t 检验和方差比 f 检验方面没有显示出显著差异。
