急性二乙基亚硝胺中毒后甲胎蛋白基因表达不受Afr2调控。

AFP gene expression after acute diethylnitrosamine intoxication is not Afr2 regulated.

作者信息

Jin David K, Anderson Eric C, Gilbert Erin, Feuerman Miriam H

机构信息

Department of Biochemistry, Box 8 SUNY Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, NY 11203, USA.

出版信息

Cancer Lett. 2005 Apr 8;220(2):211-20. doi: 10.1016/j.canlet.2004.07.031.

DOI:10.1016/j.canlet.2004.07.031

PMID:15766596

Abstract

The level of alpha-fetoprotein (AFP) gene expression during liver regeneration in mice is regulated by the Afr2 gene. C3H/HeJ mice express 10-fold higher levels of AFP than C57BL/6J mice. We show that AFP gene expression is not Afr2 regulated after intoxication with the carcinogen diethylnitrosamine (DEN). Peak levels of AFP gene expression of the 2 strains were identical, although reached at different times following treatment. Analysis of the expression of AFP derived transgenes not subject to Afr2 regulation and genetic analyses showed that the difference in timing of peak AFP gene expression after DEN intoxication was independent of Afr2 regulation.

摘要

小鼠肝脏再生过程中甲胎蛋白（AFP）基因的表达水平受Afr2基因调控。C3H/HeJ小鼠表达的AFP水平比C57BL/6J小鼠高10倍。我们发现，在用致癌物二乙基亚硝胺（DEN）中毒后，AFP基因表达不受Afr2调控。尽管两品系在处理后的不同时间达到峰值，但AFP基因表达的峰值水平是相同的。对不受Afr2调控的AFP衍生转基因表达的分析以及遗传学分析表明，DEN中毒后AFP基因表达峰值时间的差异与Afr2调控无关。