Heussen G A, Alink G M
Department of Toxicology, Agricultural University, Wageningen, The Netherlands.
Carcinogenesis. 1992 Apr;13(4):719-22. doi: 10.1093/carcin/13.4.719.
Alveolar type II cells were isolated from the lungs of female Wistar rats and were used for studies on inhibition of gap junction mediated intercellular communication (IC). Cells were incubated with 12-O-tetradecanoylphorbol-13-acetate (TPA) or extracts of airborne particulate matter (APM) and subsequently microinjected with the fluorescent dye Lucifer Yellow after which the number of fluorescent (i.e. communicating) cells was determined. Cells exposed to solvent (DMSO), showed an extensive dye coupling. Exposure of cells to TPA or extracts of APM derived from different pollution sources resulted in a strong inhibition of IC. These results show that primary cultures of rat alveolar type II cells can serve pre-eminently as a model in dye-coupling experiments. It further can be concluded that extracts of APM, in addition to the genotoxic activity that has been known for many years, also may have a tumor-promoting potency.
从雌性Wistar大鼠的肺中分离出II型肺泡细胞，并用于研究间隙连接介导的细胞间通讯（IC）的抑制作用。将细胞与12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）或空气传播颗粒物（APM）提取物一起孵育，随后用荧光染料路西法黄进行显微注射，之后确定荧光（即通讯）细胞的数量。暴露于溶剂（二甲基亚砜）的细胞显示出广泛的染料偶联。将细胞暴露于TPA或来自不同污染源的APM提取物会导致IC受到强烈抑制。这些结果表明，大鼠II型肺泡细胞的原代培养物在染料偶联实验中可以卓越地作为一种模型。还可以进一步得出结论，APM提取物除了具有多年来已知的遗传毒性活性外，还可能具有肿瘤促进潜力。
