Noshiro H, Chijiiwa K, Hirota I
Department of Surgery I, Kyushu University Faculty of Medicine, Fukuoka, Japan.
Clin Chim Acta. 1992 Feb 14;205(3):167-79. doi: 10.1016/0009-8981(92)90057-w.
To examine the hypothesis that the chemical activity of cholesterol molecules reflects the amount of cholesterol phasing out from bile associating with cholesterol monohydrate crystal nucleation, the cholesterol activities in human gallbladder biles from cholesterol gallstone patients, either untreated or treated with ursodeoxycholic acid, and from gallstone-free patients were determined in relation to the nucleation time and vesicular lipid composition. The cholesterol activity (nmol/disc/h) determined by the polyethylene disc uptake method was higher in the untreated gallstone group than the gallstone-free group (P less than 0.05) and the ursodeoxycholic acid treated group (P less than 0.01). The cholesterol activity correlated negatively with the nucleation time (P less than 0.01) and positively with both the vesicular cholesterol concentration (P less than 0.05) and the cholesterol/phospholipid ratio in vesicles (P less than 0.05). After the separation of vesicles from micelles by gel filtration, the cholesterol activity in the vesicular phase was found to be similar to that in the micellar phase. Interestingly, both the activities of cholesterol in the vesicular and micellar phases were significantly higher in the untreated gallstone group than in the gallstone-free group (P less than 0.05). These results suggest that cholesterol activity represents the amount of thermodynamically unstable cholesterol in bile.
为检验胆固醇分子的化学活性反映了从胆汁中析出并与胆固醇一水合物晶体成核相关的胆固醇量这一假说，我们测定了胆固醇结石患者未经治疗或经熊去氧胆酸治疗后的人胆囊胆汁以及无结石患者胆汁中的胆固醇活性，并将其与成核时间和囊泡脂质组成相关联。通过聚乙烯圆盘摄取法测定的胆固醇活性（nmol/圆盘/小时）在未经治疗的结石组中高于无结石组（P<0.05）和熊去氧胆酸治疗组（P<0.01）。胆固醇活性与成核时间呈负相关（P<0.01），与囊泡胆固醇浓度（P<0.05）和囊泡中的胆固醇/磷脂比值呈正相关（P<0.05）。通过凝胶过滤将囊泡与微胶粒分离后，发现囊泡相中的胆固醇活性与微胶粒相中的相似。有趣的是，未经治疗的结石组中囊泡相和微胶粒相中的胆固醇活性均显著高于无结石组（P<0.05）。这些结果表明，胆固醇活性代表了胆汁中热力学不稳定胆固醇的量。
