School of Marine Sciences, Sun Yat-sen University, Guangzhou, China.
Southern Marine Science and Engineering Guangdong Laboratory, Zhuhai, China.
Mar Biotechnol (NY). 2020 Feb;22(1):31-40. doi: 10.1007/s10126-019-09928-4. Epub 2020 Jan 2.
The yellow catfish (Pelteobagrus fulvidraco) is a very important aquaculture species distributed in freshwater area of China. All-male yellow catfish is very popular in aquaculture because of their significant sex dimorphism phenomena. The males grow much faster than females in full-sibling family. However, the sex dimorphism mechanism is still unclear in yellow catfish. In order to better understand the genetic basis of yellow catfish sexual dimorphism, it is vital to map the sex-related traits and localize the candidate genes across yellow catfish whole genome. Here, we constructed a high-density linkage map of yellow catfish using genotyping-by-sequencing (GBS) strategy. A total of 5705 single-nucleotide polymorphism (SNP) markers were mapped to 26 different linkage groups (LGs) using 184 F1 offspring. The total genetic map length was 3071.59 cM, with an average interlocus distance of 0.54 cM. Eleven significant sex-related QTLs in yellow catfish were identified. Six sex-related genes were identified from the region of reference genome near these QTLs including amh, gnrhr, vasa, lnnr1, foxl2, and bmp15. The high-density genetic linkage map provides valuable resources for yellow catfish molecular assistant breeding and elucidating sex differentiation process. Moreover, the comparative genomic study was analyzed among yellow catfish, channel catfish, and zebrafish. It revealed highly conserved chromosomal distribution between yellow catfish and channel catfish.
黄颡鱼（Pelteobagrus fulvidraco）是一种分布在中国淡水区域的重要养殖鱼类。全雄黄颡鱼因其显著的性别二态现象而在水产养殖中非常受欢迎。在全同胞家系中，雄性的生长速度明显快于雌性。然而，黄颡鱼性别二态的机制尚不清楚。为了更好地了解黄颡鱼性二态的遗传基础，对黄颡鱼全基因组进行相关性状的图谱绘制和候选基因定位是至关重要的。在这里，我们使用基于测序的基因型（GBS）策略构建了黄颡鱼的高密度连锁图谱。利用 184 个 F1 后代，共将 5705 个单核苷酸多态性（SNP）标记映射到 26 个不同的连锁群（LG）上。总遗传图谱长度为 3071.59cM，平均两基因座间距离为 0.54cM。在黄颡鱼中鉴定出 11 个显著的性别相关 QTL。在这些 QTL 附近的参考基因组区域中鉴定出 6 个性别相关基因，包括 amh、gnrhr、vasa、lnnr1、foxl2 和 bmp15。高密度遗传连锁图谱为黄颡鱼分子辅助育种和阐明性别分化过程提供了有价值的资源。此外，还对黄颡鱼、斑点叉尾鮰和斑马鱼进行了比较基因组学研究。结果表明，黄颡鱼和斑点叉尾鮰之间的染色体分布具有高度保守性。
