Wan Ling-Shu, Xu Zhi-Kang, Huang Xiao-Jun, Wang Zhen-Gang, Ye Peng
Institute of Polymer Science, Zhejiang University, Hangzhou 310027, P. R. China.
Macromol Biosci. 2005 Mar 15;5(3):229-36. doi: 10.1002/mabi.200400157.
Hemocompatibility is an essential aspect of blood contacting polymers. Knowledge of the relationship between polymer structure and hemocompatibility is important in designing such polymers. In this work, the effect of swelling behavior and states of water on the hemocompatibility of poly(acrylonitrile-co-N-vinyl-2-pyrrolidone) (PANCNVP) films was studied. Platelet adhesion and plasma recalcification time tests were used to evaluate the hemocompatibility of the films. Considering the importance of surface properties on the hemocompatibility of polymers, static water contact angles were measured by both sessile drop and captive bubble methods. It was found that, on the film surface of PANCNVP with a higher NVP content, adhered platelets were remarkably suppressed and the recalcification time was longer. The total water content adsorbed on the PANCNVP film was determined through swelling experiments performed at temperatures of interest. Differential scanning calorimetry and thermogravimetric analysis were used to probe the states of water in the films. Based on the results from these experiments, it was hypothesized that the better hemocompatibility of PANCNVP films with higher NVP contents was due to their higher free water content, because water molecule exchange at the polymer/liquid interface, facilitated by a high free water content, is unfavorable for the formation of surface bound water, which causes poor hemocompatibility. [diagram in text].
血液相容性是与血液接触的聚合物的一个重要方面。了解聚合物结构与血液相容性之间的关系对于设计此类聚合物至关重要。在这项工作中，研究了聚（丙烯腈 - 共 - N - 乙烯基 - 2 - 吡咯烷酮）（PANCNVP）薄膜的溶胀行为和水的状态对其血液相容性的影响。采用血小板黏附试验和血浆复钙时间试验来评估薄膜的血液相容性。考虑到表面性质对聚合物血液相容性的重要性，通过静滴法和俘获气泡法测量了静态水接触角。结果发现，在NVP含量较高的PANCNVP薄膜表面，黏附的血小板明显受到抑制，复钙时间更长。通过在感兴趣的温度下进行溶胀实验，测定了吸附在PANCNVP薄膜上的总含水量。采用差示扫描量热法和热重分析法来探究薄膜中水的状态。基于这些实验结果，推测NVP含量较高的PANCNVP薄膜具有更好的血液相容性是由于其较高的自由水含量，因为高自由水含量促进的聚合物/液体界面处的水分子交换不利于形成导致血液相容性差的表面结合水。[文中有图表]
